Efficient direct shoot organogenesis and genetic stability in micropropagated sacha inchi ( Plukenetia volubilis L.)
- PDF / 1,424,591 Bytes
- 7 Pages / 595.276 x 790.866 pts Page_size
- 4 Downloads / 182 Views
C Research Notes Open Access
RESEARCH NOTE
Efficient direct shoot organogenesis and genetic stability in micropropagated sacha inchi (Plukenetia volubilis L.) Catalina Restrepo‑Osorio, Alejandro Gil‑Correal, Lina Chamorro‑Gutiérrez, Viviana Ramírez‑Ríos, Javier C. Álvarez and Diego Villanueva‑Mejía*
Abstract Objective: It is necessary to improve biotech platforms based on in vitro cell tissue culture to support sacha inchi (Plukenetia volubilis L.) research programs and draw on the nutritional value of the high polyunsaturated fatty acid content of its oilseed. Here, we developed a rapid and efficient method for induction and direct in vitro shoot devel‑ opment for this species. Results: Shoots were generated from hypocotyl explants. The highest organogenic response was obtained in woody plant medium supplemented with 1 mg/L thidiazuron and 0.5 mg/L zeatin supplemented with L-glutamine, adenine hemisulfate, and L-arginine. Shoots obtained using this medium were transferred and subcultivated with different concentrations of indole-3-butyric acid and 1-naphthylacetic acid for rooting. For the first time, a histological analysis was performed supporting direct organogenic development in this species. The plantlets obtained were transferred ex vitro with a survival percentage of 80%. The genetic stability of the plants recovered was confirmed by randomly amplified polymorphic DNA analysis. All results indicate that it would be possible to stimulate direct shoot formation from hypocotyls to support the sustainable use of this species. Keywords: Adventitious shoots, Rooting, Acclimatization, Oilseed, Tissue culture Introduction Sacha inchi (Plukenetia volubilis L.) is a perennial, woody, oleaginous climbing plant belonging to the Euphorbiaceae family [1] that is native to the rainforest of South America. This crop grows in disturbed wet lowland forest at elevations of up to 900 m in the Lesser Antilles and Surinam, and along the northern and western edge of the Amazon basin in Venezuela (Amazonas), Colombia, Ecuador, Peru, Bolivia, and Brazil (western Amazonas) [2]. Its oilseed has excellent nutritional value due to its high content of polyunsaturated fatty acids, including ⍺-linolenic (C18:3, ω-3) and linoleic (C18:2, ω-6) acids *Correspondence: [email protected] Department of Biological Sciences, CIBIOP Research Group, Universidad EAFIT, Medellín, Colombia
[3], which are essential components of the human diet and help to prevent coronary heart disease and hypertension [4]. Several scientific reports have focused on regeneration from hypocotyls and epicotyls in the Euphorbiaceae family [5, 6], despite high recalcitrance. Regarding sacha inchi specifically, a few studies have reported the use of in vitro propagation to evaluate the auxin:cytokinin ratio in apical meristems [7] and different segments of the epicotyl and hypocotyl [8–10], with highest shooting but low process efficiency with 0.5 and 1 mg/L 6-benzylaminopurine and 0.1 mg/L and 0.25 mg/L 1-naphthylacetic acid (NAA). Therefore, it is imperative
Data Loading...
