DNA Nanotechnology Methods and Protocols

This second edition provides new and updated methods that detail new DNA nanotechnology techniques. Chapters focus on DNA origami nanostructures for arranging matter in the nanoscale or on their manipulation with the aid of other technologies, on procedur

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Giampaolo Zuccheri Editor

DNA Nanotechnology Methods and Protocols Second Edition

METHODS

IN

MOLECULAR BIOLOGY

Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire AL10 9AB, UK

For further volumes: http://www.springer.com/series/7651

DNA Nanotechnology Methods and Protocols Second Edition

Edited by

Giampaolo Zuccheri Department of Pharmacy and Biotechnology, University of Bologna, Bologna, Italy

Editor Giampaolo Zuccheri Department of Pharmacy and Biotechnology University of Bologna Bologna, Italy

ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-8581-4 ISBN 978-1-4939-8582-1 (eBook) https://doi.org/10.1007/978-1-4939-8582-1 Library of Congress Control Number: 2018944262 © Springer Science+Business Media, LLC, part of Springer Nature 2018 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by the registered company Springer Science+Business Media, LLC part of Springer Nature. The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.

Preface Both in research labs and in the world outside them, we are currently experiencing the mainstreaming of nanotechnology. After a few decades of discoveries and technological development of the new world at the bottom, now nanotechnology is at work in the industry as well as in many types of labs. Through mating with other sciences and technologies, it is helping to solve societal problems and to enable discoveries, often staying hidden under the hood of products and services. The mainstreaming is evident at all levels, including the goals of governments and funding bodies. Nowadays funds are granted primarily for innovation in the industry and for the solution of grand societal challenges. Nanotech can contribute to both, but