DNA Barcodes Methods and Protocols

A DNA barcode in its simplest definition is one or more short gene sequences taken from a standardized portion of the genome that is used to identify species through reference to DNA sequence libraries or databases. In DNA Barcodes: Methods and Protocols

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MOLECULAR BIOLOGY™

Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK

For further volumes: http://www.springer.com/series/7651

DNA Barcodes Methods and Protocols Edited by

W. John Kress and David L. Erickson Department of Botany, National Museum of Natural History, Smithsonian Institution, Washington, DC, USA

Editors W. John Kress, Ph.D. Department of Botany National Museum of Natural History Smithsonian Institution Washington, DC, USA

David L. Erickson, Ph.D. Department of Botany National Museum of Natural History Smithsonian Institution Washington, DC, USA

ISSN 1064-3745 ISSN 1940-6029 (electronic) ISBN 978-1-61779-590-9 ISBN 978-1-61779-591-6 (eBook) DOI 10.1007/978-1-61779-591-6 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2012931933 © Springer Science+Business Media, LLC 2012 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. Printed on acid-free paper Humana Press is part of Springer Science+Business Media (www.springer.com)

Foreword The diversity of life in a hectare of reef, a county of grassland, or a shipload of imports challenges biologists called to identify the species comprising biodiversity, functioning as ecosystems, or invading ports. The sequences of black-and-white barcodes that empower a newly hired clerk to wave a wand over a cart full of goods swiftly, print an itemized receipt infallibly, and order replacements invisibly call forth a vision of an analog for identifying species. The resemblance of barcodes on commercial products to sequences of DNA shown as black-and-white bars on electrophoretic gels reinforced the vision back in 2003 in the founding meetings of the barcode of life movement. This book edited by early adopters of DNA barcodes, John Kress and David Erickson, proves the barcode of life has arrived in environmental science. In less than a decade, they and the other authors in this volume have realized the vision of a short DNA sequence on a uniform locality of the genome to identify species rapidly and accurately. Because the currency in biology is species, their identification is no academic diversion. Biologists count the rise and fall of biodiversity in species. Regulators designate endangered species by their identified populations and reserve land where they identify the end