Dose-dependent effect of the pulsed Nd:YAG laser in the treatment of crushed sciatic nerve in Wister rats: an experiment
- PDF / 807,955 Bytes
- 10 Pages / 595.276 x 790.866 pts Page_size
- 59 Downloads / 157 Views
ORIGINAL ARTICLE
Dose-dependent effect of the pulsed Nd:YAG laser in the treatment of crushed sciatic nerve in Wister rats: an experimental model Mohamed Salaheldien Mohamed Alayat 1 & Mohammad Abubakar Basalamah 2 & Wagih Gamal Eldin Abd-Elghany Elbarrany 3 & Naser Ahmed Mahmoud El-Sawy 3 & Ehab Mohamed Abdel-Kafy 1 & Amir Abdel-Raouf El-Fiky 1 Received: 9 September 2019 / Accepted: 9 March 2020 # Springer-Verlag London Ltd., part of Springer Nature 2020
Abstract The objective of the study was to investigate the efficacy of three energy densities 4, 10, and 50 J/cm2 of pulsed Nd:YAG laser for the treatment of crushed sciatic nerve in Wister rats by evaluating changes in the sciatic functional index and the electrophysiology.A total of 180 Wistar rats were involved in the study. Rats were randomly assigned to five groups. Rats were subjected to the sciatic nerve crushing. Control negative (CONT-ve), which received no crushing; control positive (CONT+ve), which received crushing with no laser; and HILT-4, HILT-10, and HILT-50 groups, which received pulsed Nd:YAG laser (10 Hz, 360 mJ/cm2) with energy densities 4, 10, and 50 J/cm2, respectively. The SFI, the amilitude of compound motor action potential (CMAP) and sciatic motor nerve conduction velocity (MNCV) were measured before and after seven, 14, and 21 days after crushing. For the SFI and electrophysiological analysis, repeated measures ANOVA is used, followed by Bonferroni’s repeatedmeasures test. Statistical significance was set at p < 0.05. After one week, there was no significant difference in SFI, CMAP, and MNCV among the three laser groups with significant changes between them and CONT-ve and CONT+ve groups. There was a significant increase in either CMAP amplitude or MNCV after 14 days with significant decrease in the SFI after 21 days among all treatment groups. The pulsed Nd:YAG laser applied with energy densities 4, 10, and 50 J/cm2 significantly decreased the SFI and increased the CMAP and MNCV of the crushed sciatic nerve in Wister rats. Among laser doses, the difference in the rate of recovery in the electrophysiology was found after two weeks while in the SFI after three weeks. The improvement after the nerve injury was time and dose dependent.
* Mohamed Salaheldien Mohamed Alayat [email protected] Mohammad Abubakar Basalamah [email protected] Wagih Gamal Eldin Abd-Elghany Elbarrany [email protected] Naser Ahmed Mahmoud El-Sawy [email protected] Ehab Mohamed Abdel-Kafy [email protected] Amir Abdel-Raouf El-Fiky [email protected] 1
Physical Therapy Department, Faculty of Applied Medical Science, Umm Al-Qura University, Mecca, Saudi Arabia
2
Department of Pathology, Faculty of Medicine, Umm Al-Qura University, Mecca, Saudi Arabia
3
Department of Anatomy and Embryology, Faculty of Medicine, Umm Al-Qura University, Mecca, Saudi Arabia
Keywords Pulsed Nd:YAG laser . Energy density . Crushing injury . Sciatic functional index . Electrophysiology
Introduction Traumatic nerve injuries (TNI) are a common cause of motor impairme
Data Loading...
