Eco-Green Conversion of Watermelon Peels to Single Cell Oils Using a Unique Oleaginous Fungus: Lichtheimia corymbifera A
- PDF / 3,544,414 Bytes
- 12 Pages / 595.276 x 790.866 pts Page_size
- 9 Downloads / 168 Views
ORIGINAL PAPER
Eco‑Green Conversion of Watermelon Peels to Single Cell Oils Using a Unique Oleaginous Fungus: Lichtheimia corymbifera AH13 Amr Hosny Hashem1 · Mohamed Sayed Hasanin2 · Ahmed Mohamed Aly Khalil1,3 · Waleed Bakry Suleiman1 Received: 30 May 2019 / Accepted: 16 October 2019 © Springer Nature B.V. 2019
Abstract Watermelon peel waste (WPW) is being used for first time in single cell oils (SCOs) production via a promising oleaginous fungus Lichtheimia corymbifera which was isolated from Egyptian ecosystem. Pretreatments of WPW were carried out by mechanical, physical and chemical methods; the most potent pretreatment was selected according to total reducing sugar and total lipid production. Accordingly, the mechanical ptetreatment of WPW was distinctly the best pretreatment method for SCOs production from L. corymbifera. Taguchi design clarified that the most optimal culture conditions were 35 °C and pH 7.0 for 4 days by which the highest potential of SCOs and lipid content was yielded (2.93 gl−1 and 39.56% respectively). Mechanical treatment revealed that the dominant fatty acid was palmitic and oleic acids with 41.98 and 34.65% respectively with appearance of γ linolenic acid (GLA) at low concentration 1.43%. Finally, this study showed that WPW was used as a natural, effective, economic, ecofriendly and integrated substrate without adding any outsource nutrients to produce sustainable SCOs with low cost production. Graphic Abstract
Keywords Watermelon peels waste · Single cell oils · Taguchi design
Statement of Novelty * Mohamed Sayed Hasanin [email protected] Extended author information available on the last page of the article
This article includes the first report on the isolation of unique oleaginous Lichtheimia corymbifera from Egyptian ecosystem. One of the promising results in this article is decreasing the production cost of single cell oils with
13
Vol.:(0123456789)
waste management. Watermelon peels waste was used as the first report for SCOs production with minor ecofriendly pretreatment
Introduction Microbial products are described as natural products with high safety profile when compared with the risk factors correlated to chemical products which provide a harmful biproducts that may be hazardous to the ecosystem [1]. Unfortunately, the cost of production of microbial products via fermentation is relatively high, which is considered as a very limitation factor which makes it unfavorable for large scale production [2]. Microbial lipids namely SCOs are one of the microbial products that considered as a multi applications product that involved in nutraceutical, pharmaceutical and industrial fields. In medical field, SCOs are precursors for eicosanoids such as prostaglandins, leukotrienes and thromboxane, collectively known as eicosanoids, which mediate vasodilation, fever, inflammations, blood pressure, clotting, pain, neurotransmission, modulate the cholesterol biosynthesis and signaling molecules in cancer [3–6]. Moreover, SCOs play a fundamental role in maintaining normal fu
Data Loading...
