Isolation and characterization of a strain of Lichtheimia corymbifera (ex Absidia corymbifera) from a case of bovine abo
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Isolation and characterization of a strain of Lichtheimia corymbifera (ex Absidia corymbifera) from a case of bovine abortion Chiara Piancastelli, Francesca Ghidini, Gaetano Donofrio, Stefano Jottini, Simone Taddei, Sandro Cavirani and Clotilde S Cabassi* Address: Dipartimento di Salute Animale, Parma University, via del Taglio 10, 43126 Parma, Italy Email: Chiara Piancastelli - [email protected]; Francesca Ghidini - [email protected]; Gaetano Donofrio - [email protected]; Stefano Jottini - [email protected]; Simone Taddei - [email protected]; Sandro Cavirani - [email protected]; Clotilde S Cabassi* - [email protected] * Corresponding author
Published: 30 November 2009 Reproductive Biology and Endocrinology 2009, 7:138
doi:10.1186/1477-7827-7-138
Received: 24 September 2009 Accepted: 30 November 2009
This article is available from: http://www.rbej.com/content/7/1/138 © 2009 Piancastelli et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract Background: Lichtheimia corymbifera (previously Absidia corymbifera) is a filamentous zygomycetes belonging to the order Mucorales and to the family Lichtheimiaceae. Members of genus Lichtheimia spp. are cosmopolitan and ubiquitous in nature. Lichtheimia corymbifera is a recognized agent of diseases in man and animals. In cattle it causes abortion and mastitis. Three cases of bovine abortion occurred in a herd located in the Po Valley. Serological examinations were performed on fetal and mother's blood. One of the aborted fetus was referred to our laboratory. The paper describes the isolation and characterization of Lichtheimia corymbifera from a bovine aborted fetus. Methods: Serological examinations were performed on fetal and mother's blood. Lesions on fetal tissues and placenta leaded the diagnostic suspect towards a mycotic aetiology. Tissues were then put in culture, and at the same time an histological examination was performed, together with bacteriological and virological tests. The isolate from placenta and fetal tissues was identified and characterized by PCR and RFLP, using the ITS region as a target sequence and AclI restriction site within the amplicon to distinguish Lichtheimia corymbifera among the other fungi. Results: Serological, bacteriological and virological tests gave aspecific results. Histological examination evidenced numerous PAS positive hyphae within the necrotic cotiledons and numerous fungal nonseptate hyphae to the GMS stain. Colonies with typical morphological features of fungi grew up on Sabouraud agar from fetal skin and placenta. On the developed colonies the microscopic examination has shown a large number of nonseptate hyphae and sporangia consistent with Mucorales. PCR and RFLP allowed the ide
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