A Deuteromycete Isolate Geotrichum candidum as Oleaginous Cell Factory for Medium-Chain Fatty Acid-Rich Oils
- PDF / 1,507,896 Bytes
- 12 Pages / 595.276 x 790.866 pts Page_size
- 86 Downloads / 177 Views
A Deuteromycete Isolate Geotrichum candidum as Oleaginous Cell Factory for Medium‑Chain Fatty Acid‑Rich Oils Batul Diwan1 · Pratima Gupta1 Received: 22 October 2019 / Accepted: 29 July 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Single cell oils (SCO) are oils derived from microorganisms which have potential to hyperaccumulate intracellular lipids (called oleaginous) under some essential nutrient (nitrogen, phosphorous or sometimes sulphur) starvation and an excess of carbon. The present work investigates the influence of these key parameters (for triggering oleaginicity), i.e. carbon (C) and nitrogen (N) on oleaginous behaviour of an oleaginous isolate, with the objective of improving the lipid content and obtaining oils of applicative interest. Eleven yeasts were isolated from rotten fruits and a unique yeast from rotten apple was screened on the basis of its ~ 20% (of dry mass) lipid content (LC), trademark of oleaginicity under nitrogen-stressed culture conditions. Subsequent investigation on influence of C, N and w/w ratio of carbon source concentration (Cs) to nitrogen source concentration (Ns) was conducted on this isolate. The isolate was identified as a Deuteromycete—Geotrichum candidum. 4.8 g/l was found to be minimum N concentration and glucose as suitable C source for optimum balance between biomass and lipid content. The highest LC of 73.6% (172.5% higher compared to 27% LC at Cs/Ns 80/4.8) was obtained at Cs/Ns 150/4.8 with a lipid coefficient of 8.7 (g lipid/100 g substrate). While remarkably higher production economy (lipid coefficient of 28.45) was noted at Cs/Ns 100/4.8 with significant LC of 54.4% (~ 100% higher than at Cs/Ns 80/4.8). The derived oils were predominantly rich in medium-chain fatty acids (MCFA)-caprylic acid, rare in plant oils. G. candidum is a previously referred oleaginous species; however, for the first time this study illustrates its detailed oleaginous behaviour and lipid compositional characteristics with varying nutritional parameters. The work is a progressive contribution towards current and upcoming researches in field of SCOs. Compositional characteristics of derived oils, make it an important candidate for potential medical and nutritional applications in future.
Introduction Microbial species amongst bacteria, fungi and microalgae which have the capability to accumulate an excess of storage lipids (more than 20% of cell dry weight) are referred as oleaginous [1]. The accumulated oils, called single cell oils (SCO), generally contain significant amount of saturated as well as rare and nutritionally important long-chain Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00284-020-02155-4) contains supplementary material, which is available to authorized users. * Pratima Gupta [email protected]; [email protected] 1
Batul Diwan [email protected]; [email protected]
Department of Biotechnology, National Institute of Technology, Raipur, India
polyunsaturated
Data Loading...
