Effect of animal-sourced bioactive peptides on the in vitro development of mouse preantral follicles
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RESEARCH
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Effect of animal-sourced bioactive peptides on the in vitro development of mouse preantral follicles Gang Liu1,2† , Shubin Li2† , Jinyu Ren2 , Chunyu Wang2 , Yaxuan Zhang2 , Xiulan Su1* and Yanfeng Dai2*
Abstract The aim of this study was to investigate the effect of bioactive peptides (BAPT) from animal sources on the development of mouse preantral follicles in vitro. Preantral follicles were isolated and randomly divided into the following groups: an untreated group (control) and three groups supplemented with 20, 40 and 60 μg/mL BAPT, respectively. After establishing the in vitro follicle culture, the gene expression levels and hormone levels were quantified. After in vitro maturation, the developmental rates, reactive oxygen species (ROS) production levels and mitochondrial distributions of MII oocytes were investigated, followed by the analyses of embryonic developmental rates after in vitro fertilization. The results showed that BAPT promoted the growth of mouse preantral follicles. Notably, after 14 d of in vitro culture, the levels of 17 β-estradiol and progesterone were up-regulated with BAPT treatments. Moreover, the expression levels of Oct4, Bmp15, GDF9, FOXO3, Zp3, FOXL2, Inhibin alpha, SOD2, Catalase, GPx and Bcl-2 in the developing follicles were significantly up-regulated after BAPT treatments (P < 0.05), while BAPT significantly inhibited the expression levels of BAX (P < 0.05). Following BAPT treatments, the ROS production levels of MII oocytes were decreased while the mitochondrial distributions were significantly enhanced. Furthermore, increased maturation rates, fertilization and embryonic developmental rates were found in these BAPT-treated groups (P < 0.05). These results demonstrated that BAPT significantly improved the development of preantral follicles in vitro by reducing ROS-dependent cellular damages and by enhancing mitochondrial distributions, thereby promoting the further applications of animal-derived BAPT in biomedical research. Keywords: Preantral follicles, BAPT, ROS, Embryonic development
Introduction Folliculogenesis, a major issue related with mammalian ovulation and fertility, occurs as a complex and dynamic process within female ovaries, which could be affected by multiple endocrine and intraovarian * Correspondence: [email protected]; [email protected] † Gang Liu and Shubin Li contributed equally to this work. 1 Key Laboratory of Medical Cell Biology, Clinical Medicine Research Center, Affiliated Hospital of Inner Mongolia Medical College, 1 Tongdao North Street, Hohhot 010050, Inner Mongolia, China 2 College of Life Science, Inner Mongolia University, 235 West Univ. Road, Hohhot 010021, Inner Mongolia, China
paracrine factors including antioxidants, nutrients and vitamins [1]. The dysfunction of mammalian folliculogenesis, due to abnormalities in endocrine/paracrine signals, metabolic dysfunction, impaired oocyte developmental abilities or altered gene expression patterns, results in infertility-related diseases and reproductive disorders, such as ovar
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