Effect of Dietary Calcium on Adipogenesis Program and Its Role in Adipocyte Dysfunction in Male Wistar Rats
- PDF / 739,292 Bytes
- 9 Pages / 595.276 x 790.866 pts Page_size
- 49 Downloads / 205 Views
RESEARCH ARTICLE
Effect of Dietary Calcium on Adipogenesis Program and Its Role in Adipocyte Dysfunction in Male Wistar Rats Sandeep Das1 • Dipayan Choudhuri1
Received: 13 April 2019 / Revised: 13 August 2019 / Accepted: 17 August 2019 The National Academy of Sciences, India 2019
Abstract Dietary calcium influences obesity-related adipocyte dysfunction by modulating body weight. The molecular mechanism behind this weight management is under intense investigation. In the present study, the authors investigated the effect of dietary calcium on peroxisome proliferator-activated receptor-c (PPAR-c)-mediated adipogenesis program and its role in adipocyte dysfunction by measuring the inflammatory and oxidative markers within the adipose tissue of male albino rats. Healthy male rats were fed with a control diet based on AIN-93, and either lowcalcium (0.25%) or high-calcium (1.0%) diet for 90 days. A significant inverse (p \ 0.05) relationship exists between the calcium intake and anthropometric parameters like adipose tissue mass and body weight. Adipose tissue PPAR-c activity along with its downstream enzyme activities like glyceraldehydes 3 phosphate dehydrogenase, lipoprotein lipase as well as the protein expression of fatty acid-binding protein 4 and calcium-sensing receptor were significantly increased (p \ 0.05) in the low-calcium-fed rats which in turn promoted the adipogenesis program. At the same time, protein expression of inflammatory markers like tumor necrosis factor-a and interleukin-6 and oxidative stress markers like lipid peroxidation and protein carbonylation were significantly increased (p \ 0.05) in low-calcium-fed Significant Statement Dietary calcium prevents obesity but the molecular mechanism was not clear. So the present study shows that dietary calcium affects the PPAR-c-mediated adipogenesis program which is related to the risk of obesity and adipocyte dysfunction. & Dipayan Choudhuri [email protected] 1
Reproductive Physiology and Endocrinology Laboratory, Department of Human Physiology, Tripura University (A Central University), Suryamaninagar, Agartala, Tripura 799022, India
rats. These deleterious effects were suppressed by the highcalcium diet by preventing the activation of the adipogenesis program. Thus, the present study showed the involvement of dietary calcium against the PPAR-c-mediated adipogenesis program as well as its role in adipocyte dysfunction by regulating the inflammatory and oxidative stress. Keywords Calcium PPAR-c Adipogenesis Inflammation Oxidative stress
Introduction The excessive growth of adipocytes through cellular differentiation during obesity is considered to be an emerging global health disorder which is associated with various comorbid conditions. The maturation of newly formed adipocytes, the major cellular component of adipose tissue seemed to be regulated by several factors like genetic, metabolic, neurological, pharmacological and nutritional factors [1]. The expansion of adipose tissue through adipogenesis program results in the for
Data Loading...
