Effect of freeze-drying and gamma irradiation on biomechanical properties of bovine pericardium
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Springer 2005
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Effect of freeze-drying and gamma irradiation on biomechanical properties of bovine pericardium Y.M. Hafeez1, A.B.Z. Zuki2,*, N. Yusof3, H. Asnah3, M.Y. Loqman2, M.M. Noordin2 and M.Y. Ainul-Yuzairi2 1
Faculty of Veterinary Medicine, University of Khartoum, P.O. Box 32 Shambat, Khartoum North, Sudan; Faculty of Veterinary Medicine, Universiti Putra Malaysia 43400 UPM, Serdang, Selangor Darul Ehsan, Malaysia; 3Malaysian Institute for Nuclear Technology Research (MINT) Bangi, 43000 Kajang, Selangor Darul Ehsan, Malaysia; *Author for correspondence (e-mail: [email protected]) 2
Received 14 January 2004; accepted in revised form 5 August 2004
Key words: Biomechanical properties, Freeze-dried bovine pericardium, Gamma irradiation
Abstract Freeze-drying and gamma irradiation are the techniques widely use in tissue banking for preservation and sterilization of tissue grafts respectively. However, the effect of these techniques on biomechanical properties of bovine pericardium is poorly known. A total of 300 strips of bovine pericardium each measured 4 cm · 1 cm were used in this study to evaluate the effect of freeze-drying on biomechanical properties of fresh bovine pericardium and the effect of gamma irradiation on biomechanical properties of freeze-dried bovine pericardium. The strips were divided into three equal groups, which consist of 100 strips each group. The three groups were fresh bovine pericardium, freeze-dried bovine pericardium and irradiated freeze-dried bovine pericardium. The biomechanical properties of the pericardial strips were measured by a computer controlled instron tensiometer while the strips thickness was measured by Mitutoyo thickness gauge. The results of the study revealed that freeze-drying has no significant (p > 0.05) effect on the tensile strength, Young’s modulus (stiffness) and elongation rate of fresh bovine pericardium. Irradiation with 25 kGy gamma rays caused significant decreased in the tensile strength, Young’s modulus and elongation rate of the freeze-dried pericardium. However, gamma irradiation has no significant effect on the thickness of freeze-dried bovine pericardium, while freeze-drying caused significant decreased in the thickness of the fresh bovine pericardium. The outcome of this study demonstrated that freeze-drying has no significant effect on the biomechanical properties of fresh bovine pericardium, and gamma irradiation caused significant effect on the biomechanical properties of freezedried bovine pericardium.
Introduction Freeze-drying which includes rapid freezing and dehydration of the frozen product under high vacuum is commonly used for preparation of a stable biological product that can be stored at
room temperature. Freeze-drying as an industrial process dates from the time of Second World War when the demand for human blood plasma reached critical proportion (Flanks 1998). The advantages of freeze-drying in clinical practice include decreasing of surgical time and surgical
86 morbidity, assessment of the graft size and shape prior to operatio
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