Effect of Lactobacillus rhamnosus NCDC 298 with FOS in Combination on Viability and Toxin Production of Enterotoxigenic
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Effect of Lactobacillus rhamnosus NCDC 298 with FOS in Combination on Viability and Toxin Production of Enterotoxigenic Escherichia coli Santosh Anand 1 & Surajit Mandal 1 & Sudhir Kumar Tomar 1
# Springer Science+Business Media, LLC 2017
Abstract The present study was to investigate the utilization of prebiotics by Lactobacillus rhamnosus NCDC 298 and its synergistic adversary effect on both population and production of heat-labile (LT) toxin in enterotoxigenic Escherichia coli (ETEC). To select suitable prebiotic in order to enhance functionality, its utilization and the prebiotic activity score was examined. Antivirulence effect on ETEC was inspected by its inactivation rate and heat-labile toxin production in presence of different synbiotic combination. L. rhamnosus NCDC 298 strain grown well on media supplemented with fructooligosaccharides (FOS) and galactooligosaccharides (GOS), whereas significant inactivation of ETEC was observed when FOS was added to the co-culture medium. Significant decrease in LT enterotoxin was seen through GM1 ganglioside enzyme linked immunoassay (GM1 ELISA), when ETEC has grown with L. rhamnosus NCDC 298 and FOS. Short-chain FOS proved to be the most effective substrate, improving antagonistic activity for L. rhamnosus NCDC 298. Both L. rhamnosus NCDC 298 with FOS can be used as an effective synbiotic combination for secretory antidiarrheal fermented dairy formulations.
Keywords Probiotics . Enterotoxigenic Escherichia coli . Prebiotics . Synbiotic . Diarrhea
* Surajit Mandal [email protected]
1
TFSL, Dairy Microbiology Division, ICAR-National Dairy Research Institute, Karnal, Haryana, India
Introduction Enterotoxigenic Escherichia coli (ETEC) is one of the potential pathogenic prototype strains of E. coli. This pathogenic strain is found to be a major causative agent of traveler’s and acute diarrhea with 40–70% of the cases worldwide [1]. ETEC is estimated to cause 280–400 million diarrheal episodes annually in children less than 5 years of age, resulting in 300,000 to 500,000 deaths. ETEC is the main causative agent of traveler’s diarrhea (TD) and acute diarrhea in children. ETEC is the second leading cause of death in children less than 5 years of age [2]. ETEC colonize the surface of the small bowel mucosa and elaborate their enterotoxins, giving rise to a secretive state. ETEC strains cause diarrhea through the action of their enterotoxins. ETEC strains may express a heat-labile toxin (LT) only, heat stable toxin (ST) only, or both LT and ST. ETEC invade colonic epithelium through intracellular multiplication after penetration and lysis of endocytic vacuoles to cause secretory diarrhea. Probiotics are Blive microorganisms which when administered in adequate amounts confer a health benefit on the host^ [3]. In luminal acting class, live strains of microorganism based probiotic therapy gained a role as an adjunctive treatment [4]. Probiotics act as natural potential agents which can restrict pathogens and prevent dysbiosis by influencing gut microbiota in a manner
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