Effect of microwave-assisted deep eutectic solvent pretreatment on lignocellulosic structure and bioconversion of wheat
- PDF / 1,173,655 Bytes
- 14 Pages / 547.087 x 737.008 pts Page_size
- 27 Downloads / 224 Views
(0123456789().,-volV) ( 01234567 89().,-volV)
ORIGINAL RESEARCH
Effect of microwave-assisted deep eutectic solvent pretreatment on lignocellulosic structure and bioconversion of wheat straw Asli Isci . Gizem Melissa Erdem . Simel Bagder Elmaci . Ozge Sakiyan . Anne Lamp . Martin Kaltschmitt
Received: 15 May 2020 / Accepted: 28 July 2020 Ó Springer Nature B.V. 2020
Abstract In this study, it was aimed to investigate the efficacy of microwave assisted deep eutectic solvent (MW-DES) pretreatment on the structure of wheat straw as well as the sugar and ethanol yields. The samples were treated with choline chloride: formic acid at different mole ratios (1:2, 1:3, and 1:4), microwave power (270, 360, and 450 W) and pretreatment time (2, 5, and 8 min). MW-DES pretreatment has a significant impact on lignocellulosic structure, which was also verified by XRD and SEM analysis. It was found that xylan is the most affected constituent of biomass during MW-DES pretreatment. Severe conditions can solubilize up to 90% of the xylan into liquid phase. The removal of xylan and lignin during MW-DES pretreatment led to highly digestible and fermentable wheat straw fibers.
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10570-020-03371-8) contains supplementary material, which is available to authorized users. A. Isci (&) G. M. Erdem S. Bagder Elmaci O. Sakiyan Faculty of Engineering, Food Engineering Department, Ankara University, Go¨lbasi, Ankara, Turkey e-mail: [email protected]
Enzymatic hydrolysis of pretreated wheat straw fibers revealed that MW-DES pretreatment was vastly effective at achieving very high sugar yields (99% glucose, 85% xylose yield). A linear correlation between xylan removal and glucose release during enzymatic hydrolysis was observed. The maximum total sugar release (619 mg/g pretreated wheat straw fibers) being twice as much of conventional deep eutectic solvent (DES) pretreatment, was observed at 1:3 mol ratio, 360 W microwave power and 8 min retention time. The theoretical ethanol yield using Escherichia coli KO11 was 81.5%. Keywords Wheat straw Deep eutectic solvent Microwave Pretreatment Bioethanol Abbreviations CrI Crystallinity index DES Deep eutectic solvent MWMicrowave assisted deep eutectic solvent DES SEM Scanning electron microscope SSCF Simultaneous saccharification and cofermentation SR Solid recovery XRD X-ray diffractometry
A. Isci A. Lamp M. Kaltschmitt Institute of Environmental Technology and Energy Economics (IUE), Hamburg University of Technology (TUHH), Eissendorfer Straße 40, 21073 Hamburg, Germany
123
Cellulose
Introduction In today’s era, economic stability and national security issues are tightly linked to energy security. The crude oil consumption not only causes environmental problems and contributes to global climate change but also creates national security issues. Thus, it is necessary to replace fossil fuels by domestic renewable and sustainable sources of energy. Lignocellulosic material such as agricul
Data Loading...
