Effect of Plant Growth Regulators and Additives on Indirect Organogenesis of Simarouba glauca DC
A protocol of in vitro propagation of Simarouba glauca DC (Simaroubaceae) by indirect organogenesis under the influence of different combinations of plant growth regulators (PGRs) was standardized. Indole-3-butyric acid 1.0 mg/l produced the highest callu
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Effect of Plant Growth Regulators and Additives on Indirect Organogenesis of Simarouba glauca DC A.R. Lavanya, M. Muthukumar, S. Muthukrishnan, V. Kumaresan, T. Senthil Kumar, M. Vijaya Venkatesh, and M.V. Rao
Abstract
A protocol of in vitro propagation of Simarouba glauca DC (Simaroubaceae) by indirect organogenesis under the influence of different combinations of plant growth regulators (PGRs) was standardized. Indole-3-butyric acid 1.0 mg/l produced the highest callusing response: 100 % in cotyledon and 95 % in internode explant. One hundred percent of shoot bud induction response with shoot number 18.0 for cotyledon and 85 % response of shoot bud induction with shoot number 12.5 for internode were observed in 6-benzyl adenine (BA) 3.0 mg/l, 6-furfuryl amino purine 0.5 mg/l, and GA31.0 mg/l. Organic additive coconut water that resulted in 95 % response with average 25.0 shoot number for cotyledon and 80 % response with average shoot number of 18.2 for internode was observed
A.R. Lavanya Department of Plant Science, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India Department of Botany, Periyar E. V. R. Govt. Arts College, Tiruchirappalli 620 023, Tamil Nadu, India M. Muthukumar • S. Muthukrishnan • M. Vijaya Venkatesh • M.V. Rao (*) Department of Plant Science, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India e-mail: [email protected] V. Kumaresan Department of Plant Science, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India Department of Botany, Aringar Anna Govt. Arts College, Attur, Salem 636 121, Tamil Nadu, India T. Senthil Kumar Department of Industry University Collaboration, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India © Springer Science+Business Media Singapore 2016 M. Anis, N. Ahmad (eds.), Plant Tissue Culture: Propagation, Conservation and Crop Improvement, DOI 10.1007/978-981-10-1917-3_4
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in the medium containing CW (15 %). GA3 promoted only shoot multiplication with no shoot elongation. High concentration of GA3 directly involved in shoot elongation rather multiplication, but it caused some irreversible root inhibition. α-Naphthalene acetic acid 2.0 mg/l showed 95 % and 80 % of rooting response with root numbers 8.5 and 7.2 in cotyledon and internode explants, respectively. The microshoots with the lengthy roots were transferred to paper cups for acclimatization. After a period of 12 weeks, acclimatized plants were successfully transferred to the field. The 56.8 % plants survived in the field. Keywords
Paradise tree • Simarouba glauca DC • Organogenesis • Microshoots • GA3 • Coconut water
4.1
Introduction
Simarouba glauca DC (Simaroubaceae), commonly known as paradise tree, is a fast-growing, multipurpose, medium-sized tree that grows up to 20 m high, with a trunk 50–80 cm in diameter. It is indigenous to the Amazon rain forest and other tropical areas in Mexico, Cuba, Haiti, Jamaica, and Central America (Cronquist 1944; Armour 1959). It can grow at elevations from sea level to
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