Effect of rhamnolipid on the physicochemical properties and interaction of bacteria and fungi
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FUNGAL AND BACTERIAL PHYSIOLOGY - RESEARCH PAPER
Effect of rhamnolipid on the physicochemical properties and interaction of bacteria and fungi Nurhidayah Hamzah 1 & Norhafezah Kasmuri 1
&
Wei Tao 2 & Naresh Singhal 3 & Lokesh Padhye 3 & Simon Swift 4
Received: 25 August 2019 / Accepted: 7 May 2020 # Sociedade Brasileira de Microbiologia 2020
Abstract Bacterial adhesion on surfaces is an essential initial step in promoting bacterial mobilization for soil bioremediation process. Modification of the cell surface is required to improve the adhesion of bacteria. The modification of physicochemical properties by rhamnolipid to Pseudomonas putida KT2442, Rhodococcus erythropolis 3586 and Aspergillus brasiliensis ATCC 16404 strains was analysed using contact angle measurements. The surface energy and total free energy of adhesion were calculated to predict the adhesion of both bacteria strains on the A. brasiliensis surface. The study of bacterial adhesion was carried out to evaluate experimental value with the theoretical results. Bacteria and fungi physicochemical properties were modified significantly when treated with rhamnolipid. The adhesion rate of P. putida improved by 16% with the addition of rhamnolipid (below 1 CMC), while the increase of rhamnolipid concentration beyond 1 CMC did not further enhance the bacterial adhesion. The addition of rhamnolipid did not affect the adhesion of R. erythropolis. A good relationship has been obtained in which water contact angle and surface energy of fungal surfaces are the major factors contributing to the bacterial adhesion. The adhesion is mainly driven by acid-base interaction. This finding provides insight to the role of physicochemical properties in controlling the bacterial adhesion on the fungal surface to enhance bacteria transport in soil bioremediation. Keywords Acid-base interactions . Biological adhesion . Physicochemical properties . Rhamnolipid . Water contact angle
Introduction Soil bioremediation strategy is limited to the physical access of bacteria in targeting pollutants. Bacteria mobilization is restricted to access porous soil as it contains less water present and has higher air-water interface, which would influence bacteria Responsible Editor: Rosane Freitas Schwan. * Norhafezah Kasmuri [email protected] 1
Department of Water Resources and Environmental Systems, Faculty of Civil Engineering, Universiti Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia
2
School of Chemistry and Environmental Engineering, Sichuan University of Science and Engineering, Zigong 643000, People’s Republic of China
3
Department of Environmental Engineering, Faculty of Civil and Environmental Engineering, University of Auckland, Auckland, New Zealand
4
Department of Molecular Medicine and Pathology, University of Auckland, Auckland, New Zealand
accumulation [1]. Many studies were conducted to improve bacteria migration, including electrophoresis [2], environmental control [3–5], polymers [6] and bio-accessor (earthworm, chemotactic bacteria and fungi)
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