Effect of transgenic cotton continuous cropping on soil bacterial community
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Tian et al. Annals of Microbiology (2020) 70:61 https://doi.org/10.1186/s13213-020-01602-8
ORIGINAL ARTICLE
Open Access
Effect of transgenic cotton continuous cropping on soil bacterial community Wen-hui Tian1†, Xiao-long Yi1†, Shan-shan Liu1,2†, Chao Zhou1 and Ai-ying Wang1*
Abstract Purpose: In agricultural practices, continuous cultivation of genetically modified crops with high commercial value has a definite impact on soil microbial diversity. Soil microorganisms directly define the operational degree and function realization of the soil ecosystem. To understand the safety of environmental release, we studied the effects of continuous cropping of transgenic cotton on the diversity of bacterial communities in the rhizosphere soil. Methods: We have applied a high-throughput sequencing method and compared the bacterial community structure as well as diversity of rhizosphere soil of the transgenic cotton line (25C-1) and its parent cotton line (TH2). Result: Structural analysis of the bacterial community showed that Arthrobacter and Sphingomonas are significantly enriched after continuous cropping of transgenic cotton lines and had a positive impact on the soil’s ecological environment. Interestingly, parameters of the physical and chemical properties of soil used for the continuous cropping of the two cotton lines for 3 consecutive years show no detectable change, other than total nitrogen. Notably, Spearman’s correlation analysis suggests that total nitrogen is the key environmental factor that affects the bacterial community of the soil used to cultivate the transgenic cotton. Conclusion: We did not find a notable difference in species diversity between the two samples. However, the proportions of beneficial bacteria (Arthrobacter and Sphingomonas) increased and the total nitrogen content has changed in 3 years. These results provide necessary insights into the function and role of bacteria in transgenic cotton. This study will help future investigators assess the potential ecological risks of genetically modified plants. Keywords: Transgenic cotton rhizosphere soil, High-throughput sequencing, Bacterial community, Environmental safety assessment of transgenic crops
Introduction The global production of genetically modified crops has grown profoundly from 1.7 million hectares in 1996 to 191.7 million hectares in 2018 (Babar et al. 2019). The agriculture practices with commercial transgenic crops, such as cotton (a leading cash crop) and maize (Trivedi et al. 2012), have brought enormous economic benefits worldwide (Guo et al. 2018). However, world average cotton yield in 2005 was estimated at 650 kg lint ha−1, a 73% loss to various stresses (comparable to those values * Correspondence: [email protected] † Wen-hui Tian, Xiao-long Yi and Shan-shan Liu contributed equally to this work. 1 College of Life Sciences, Shihezi University, Shihezi 832000, China Full list of author information is available at the end of the article
published for other crops). Among all stresses, the main factors affecting cotton yield
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