Effects of biomimetic micropattern on titanium deposited with PDA/Cu and nitric oxide release on behaviors of ECs
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Effects of biomimetic micropattern on titanium deposited with PDA/Cu and nitric oxide release on behaviors of ECs Wenyong Ma1 Nan Huang2
, Luying Liu2, Xiao luo2, Congzhen Han2, Ping Yang2,a), Yuancong Zhao2,b),
1
Key Laboratory of Advanced Technology of Materials, Ministry of Education, School of Material Science and Engineering, Southwest Jiaotong University, Chengdu 610031, People’s Republic of China; and Material Technology Insitution, Yibin University, Yibin 644000, People’s Republic of China 2 Key Laboratory of Advanced Technology of Materials, Ministry of Education, School of Material Science and Engineering, Southwest Jiaotong University, Chengdu 610031, People’s Republic of China a) Address all correspondence to these authors. e-mail: [email protected] b) e-mail: [email protected] Received: 17 October 2018; accepted: 22 April 2019
Surface modification with poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) is an effective method for improving hemocompatibility. Peptide GREDVY immobilized on Ti is of great benefit to endothelialization. Micropattern of PMPC and GREDVY can regulate cells distribution, behaviors, and nitric oxide (NO) release. Copper can be used as catalytic to release NO from a donor in vitro, which can inhibit platelets adhesion, activation, and aggregation. The Ti-PDA(Cu)-M/R(P) micropattern was fabricated with PMMPC-HD {PMMPC [monomer contain MPC and methacrylic acid (MA)] was cross-linked with hexamethylene diamine} and peptide Gly-Arg-Glu-Asp-Val-Tyr (GREDVY) using PDMS stamp, and it was characterized by SEM, FTIR, and XPS. The results demonstrated that the PMPC and peptide GREDVY were immobilized onto polydopamine successfully. Simultaneously, the copper existed in polydopamine was confirmed by XPS. The rate of NO release in vitro catalyzed by copper ions was 1.5–5.3 × 10−10 mol/(cm2 min). It will be beneficial to inhibiting platelets adhesion and proliferation of ECs.
Introduction The principal requirement for biomaterials is biocompatibility, and an ideal biocompatible biomaterial should perform its function without causing undue host response or resulting adverse clinical reaction [1, 2]. As regards cardiovascular implants that contact blood directly, such as pacemaker components, heart valves, and endovascular stents, blood compatibility is the first consideration. To improve blood compatibility, anticoagulant (heparin, bivalirudin, hirudin, etc.) and zwitterionic (materials that possess moieties with both cationic and anionic groups, such as MPC and glycine betaine) polymers were immobilized on a substrate. Endothelialization of the modified surface needs to be further improved. Recently, nitric oxide (NO) as an important biological signal molecule was used to improve hemocompatibility and endothelial cells (ECs) compatibility [3]. Systemic use of NO in studies related to thrombosis has led to the consensus that NO prevents platelet adhesion and activation,
ª Materials Research Society 2019
and similar in response to anticoagulant agents (heparin and hirudin) without the long
