Effects of growth conditions of donor plants and in vitro culture environment in the viability and the embryogenic respo
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Effects of growth conditions of donor plants and in vitro culture environment in the viability and the embryogenic response of microspores of different eggplant genotypes Alba Rivas-Sendra . Patricia Corral-Martı´nez . Carolina Camacho-Ferna´ndez . Rosa Porcel . Jose M. Seguı´-Simarro
Received: 11 August 2020 / Accepted: 28 September 2020 Ó Springer Nature B.V. 2020
Abstract Notwithstanding the importance of eggplant in global horticulture, doubled haploid production in this species is still far from being efficient. Although acknowledged to have a role in the efficiency of androgenesis induction, factors such as the growth conditions of donor plant or the in vitro culture environment have not been deeply explored or not explored at all in eggplant, which leaves room for further improvement. In this work, we investigated the effects of different in vivo and in vitro parameters on the androgenic performance of different eggplant genotypes, including two hybrids and a DH line. The in vivo parameters included the exposure of donor plants to different temperature and light conditions and to increased levels of boron. The in vitro parameters included the use of different concentrations of NLN medium components, sucrose and growth regulators, and the suspension of microspores at different densities. Our results showed that whereas greenhouse temperature variations or boron application did not to have a positive influence, greenhouse lighting influenced their viability, thereby conditioning the Alba Rivas-Sendra and Patricia Corral-Martı´nez have contributed equally to this work. A. Rivas-Sendra P. Corral-Martı´nez C. Camacho-Ferna´ndez R. Porcel J. M. Seguı´-Simarro (&) Cell biology Group - COMAV Institute, Universitat Polite`cnica de Vale`ncia, Valencia, Spain e-mail: [email protected]
embryogenic response. Changes in different sucrose, salts and hormone levels had different effects in the genotypes studied, which correlated with their genetic constitution. Finally, we determined the best microspore density, different from that previously proposed. Our work shed light on the role of different factors involved in eggplant microspore cultures, some of them not yet studied, contributing to make microspore culture a more efficient tool in eggplant breeding. Keywords Androgenesis Growth regulators Microspore culture Microspore embryogenesis Solanum melongena L.
Introduction In microspore embryogenesis, microspores are switched from microsporogenesis towards an alternative in vitro pathway where they develop as haploid or doubled haploid (DH) individuals. After specific in vitro stress treatments, some microspores are insensitive to it and follow a gametophytic-like developmental program (Satpute et al. 2005), but other microspores are successfully induced to divide sporophytically, becoming haploid or DH embryos. DH technology is a convenient tool in plant breeding, since it produces fully homozygous plants (pure lines) in a reduced period of time and with
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