Effects of high metal concentrations on antioxidative system in Phragmites australis grown in mine and flotation tailing

  • PDF / 604,507 Bytes
  • 16 Pages / 547.087 x 737.008 pts Page_size
  • 44 Downloads / 192 Views

DOWNLOAD

REPORT


REGULAR ARTICLE

Effects of high metal concentrations on antioxidative system in Phragmites australis grown in mine and flotation tailings ponds Milijana Kovačević & Živko Jovanović & Gordana Andrejić & Željko Dželetović & Tamara Rakić

Received: 25 December 2019 / Accepted: 8 June 2020 # Springer Nature Switzerland AG 2020

Abstract Aims Phragmites australis grows as a pioneer plant species in several mine and flotation tailings ponds distinguished by extremely high concentrations of metals. The main goals of this study were to estimate the effects of the specific concentrations and combinations of accumulated metals on the efficiency of antioxidative enzymes and plant oxidative status. This study is relevant to our understanding of the common reed exceptional capacity to endure extreme edaphic conditions. Methods Metal concentrations were determined in the sediment, roots and leaves. Antioxidative enzymes activities, amounts of pigments and phenolics, total Responsible Editor: Antony Van der Ent.

antioxidative capacity (TAC), lipid peroxidation level (LP) were analysed in plant organs. Results Effects of accumulated metals depended on their concentrations and their stoichiometry. Antioxidative enzymes and TAC in roots were significantly reduced, resulting in consequent increase in LP. Pb concentration in leaves did not significantly change enzymes activities, whereas toxic level of Cu impeded activity of catalaze and ascorbate peroxidase. Conclusions The results indicate that in the conditions of high root metal contamination the mechanisms involved in their immobilization and detoxification cannot completely restrain their toxicity. Their effects on enzymes activities depend on the type of enzyme, metal concentrations, specific ratios and interactions.

Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11104-020-04598-x) contains supplementary material, which is available to authorized users.

Keywords Common reed . Metal antagonism . Metal tolerance . Oxidative stress . Phytostabilization

M. Kovačević : T. Rakić (*) Faculty of Biology, Department of Plant Ecology and Phytogeography, University of Belgrade, Belgrade, Serbia e-mail: [email protected]

Abbreviations APX ascorbate peroxidase CAT catalase Chl chlorophyll Chl (a + b) total chlorophyll Chl a/b Chl a / Chl b ratio Car total carotenoids GR glutathione reductase LP lipid peroxidation level POD total soluble peroxidases

Ž. Jovanović Faculty of Biology, Department of Biochemistry and Molecular Biology, University of Belgrade, Belgrade, Serbia G. Andrejić : Ž. Dželetović Institute for the Application of Nuclear Energy, University of Belgrade, Belgrade, Serbia

Plant Soil

SOD TAC Ph

superoxide dismutase total antioxidative capacity phenolics

Introduction Metal exploitation has been increased with the permanent expansion of metal application in industry. Although metal mining increases economic prosperity, the enormous amounts of mine tailings deposited over the large areas of natural vegetation cause the

Data Loading...

Recommend Documents