Aeromicrobium endophyticum sp. nov., an endophytic actinobacterium isolated from reed ( Phragmites australis )
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Aeromicrobium endophyticum sp. nov., an endophytic actinobacterium isolated from reed (Phragmites australis)§ Fei-Na Li1, Shui-Lin Liao2,3,4, Shao-Wei Liu1, Tao Jin2,3, and Cheng-Hang Sun1*
Keywords: endophytic actinobacterium, Aeromicrobium endophyticum, genome comparison, single-copy phylogenetic marker genes
1
Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, P. R. China 2 BGI-Shenzhen, Beishan Industrial Zone, Shenzhen 518083, P. R. China 3 China National GeneBank, BGI-Shenzhen, Shenzhen 518120, P. R. China 4 BGI Education Center, University of Chinese Academy of Sciences, Shenzhen 518083, P. R. China (Received Dec 27, 2018 / Revised Mar 21, 2019 / Accepted Apr 4, 2019)
A Gram-staining-positive, motile and short-rod-shaped actinobacterium designated 9W16Y-2T was isolated from surface-sterilized leaves of reed (Phragmites australis) collected from Taklamakan Desert in Xinjiang Uygur Autonomous Region, China. Colonies were pale greenish yellow, circular, smooth, and convex. The 16S rRNA gene sequence of strain 9W16Y-2T exhibited highest sequence similarities with Aeromicrobium camelliae CGMCC 1.12942T (99.0%) and AeroT microbium erythreum NRRL B-3381 (97.2%). Phylogenetic analyses based on 16S rRNA gene sequences and single-copy phylogenetic marker genes (pMGs) showed that strain 9W16YT 2 belonged to the genus Aeromicrobium and formed a monophyletic clade with Aeromicrobium camelliae CGMCC 1.12942T. Furthermore, average nucleotide identity (ANI) and DNA-DNA hybridization (DDH) clearly separated strain 9W16Y-2T from the other species of the genus Aeromicrobium with values below the thresholds for species delineation. The G+C content of the genomic DNA is 68.9 mol%. The diagnostic diamino acid of the cell-wall peptidoglycan was LLdiaminopimelic acid. The predominant menaquinone was MK-9(H4). The major fatty acids (> 10% of the total fatty acids) were C18:0 10-methyl (TBSA) (28.2%), C16:0 (21.0%), C16:0 2-OH (20.8%) and C18:1 ω9c (12.8%). The polar lipid profile comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylinositol, an unidentified aminophospholipid and an unidentified lipid. Based on the phylogenic, phenotypic and chemotaxonomic features, strain 9W16Y-2T represents a novel species of the genus Aeromicrobium, for which the name Aeromicrobium endophyticum sp. T nov. is proposed. The type strain is 9W16Y-2 (= CGMCC T T 1.13876 = JCM 33141 ).
*For correspondence. E-mail: [email protected]; Tel: +86-1063165278; Fax: +86-10-63017302 § Supplemental material for this article may be found at http://www.springerlink.com/content/120956. Copyright G2019, The Microbiological Society of Korea
Introduction The genus Aeromicrobium was affiliated to the family Nocardioidaceae (Nesterenko et al., 1985) of the order Propionibacteriales and first proposed by Miller et al. (1991) with Aeromicrobium erythreum as the type species in 1991. Subsequently Nocardioides fastidiosa wa
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