Comparative pharmacologic profiles of leaves and hypocotyls of a mangrove plant: Bruguiera gymnorrhiza
- PDF / 933,404 Bytes
- 9 Pages / 595.276 x 790.866 pts Page_size
- 83 Downloads / 165 Views
RESEARCH ARTICLE
Comparative pharmacologic profiles of leaves and hypocotyls of a mangrove plant: Bruguiera gymnorrhiza Mimi Golder1 · Samir Kumar Sadhu1 · Biswajit Biswas1,2 · Tannami Islam1 Received: 16 September 2019 / Accepted: 15 October 2019 © Institute of Korean Medicine, Kyung Hee University 2020
Abstract Ethanolic extracts of leaves and hypocotyls of a mangrove plant, Bruguiera gymnorrhiza (Family Rhizophoraceae), were investigated to identify the presence of different phytochemicals and to assess the antihyperglycemic, antidiarrheal, analgesic and antioxidative activity in the current study. Oral glucose tolerance test (OGTT) was performed to investigate the in vivo antihyperglycemic activity and leaves extract (LE) was found to possess significant antihyperglycemic property compared to hypocotyl extract (HE). Therefore, the leaves extract was considered for α-Glucosidase enzyme inhibitory activity test and substantial α-glucosidase enzyme inhibitory property was observed with an IC50 (concentration required to inhibit 50% enzyme) value of 2.670. Antioxidative activity of plant extracts was tested in vitro by evaluating their ability to scavenge DPPH, hydrogen peroxide and hydroxyl radical as well as superoxide anions. In all cases, LE showed greater activity than HE. In addition, LE demonstrated higher amount of total phenolics, flavonoids and tannins indicating higher antioxidant effect compared to HE. For the evaluation of antidiarrheal effect in castor oil induced diarrheal mice, both extracts showed significant inhibition of defecation at doses 250 and 500 mg/kg body weight. Finally, for acetic acid-induced writhing method to evaluate the analgesic activity, significant percentage inhibition of writhing (P 500 µg/ml and 66.52 µg/ml, respectively, whereas for standard ascorbic acid, the S C50 value was 14.42 µg/ml. For hydrogen peroxide scavenging activity test, the S C50 value for ascorbic acid standard, HE and LE was 83.95 µg/ ml, 210.38 µg/ml and 115.88 µg/ml, respectively (Table 1).
Table 1 SC50 values of different scavenging assays of sample extracts Sample extract
DRSA (SC50 µg/ mL)
HPSA (SC50 µg/ ml )
HRSA (SC50 µg/ ml )
SRSA (SC50 µg/ ml )
LE HE
66.52 500
115.88 210.38
87.49 160.32
124.74 229.61
DRSA (DPPH Radical Scavenging Activity), HPSA (Hydrogen Peroxide Scavenging Activity), HRSA (Hydroxyl Radical Scavenging Activity), SRSA (Superoxide Radical Scavenging Activity)
13
M. Golder et al.
In case of the LE, the S C50 value in DPPH and hydrogen peroxide radical scavenging activity test corresponds to a previous study (Mahmud et al. 2017). While investigating the hydroxyl radical scavenging activity, both LE & HE showed peroxyl radical scavenging property in a concentration dependent manner with an SC50 value of 87.49 and 160.32 µg/ml, respectively, while for ascorbic acid standard, the SC50 value was 62.95 (Table 1). Finally, in case of the superoxide radical scavenging activity test, LE displayed superior superoxide radical scavenging activity with an S C50 v
Data Loading...
