Efficacy of novel nano-hydroxyapatite/polyurethane composite scaffolds with silver phosphate particles in chronic osteom
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TISSUE ENGINEERING CONSTRUCTS AND CELL SUBSTRATES Original Research
Efficacy of novel nano-hydroxyapatite/polyurethane composite scaffolds with silver phosphate particles in chronic osteomyelitis Dongli Zhang1 Wen Liu1 Xiang-Dong Wu1 Xiaoqiang He1 Xiao Lin1 Han Wang1 Jidong Li2 Jiaxing Jiang2 Wei Huang 1 ●
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Received: 13 March 2018 / Accepted: 6 May 2019 / Published online: 24 May 2019 © Springer Science+Business Media, LLC, part of Springer Nature 2019
Abstract Recently, chronic osteomyelitis is still a challenging surgical problem. Unfortunately, the traditional clinical method using bone cement loaded antibiotics is restricted due to its non-biodegradability and limited release of antibiotics. Hydroxyapatite is a good adsorbent with good biocompatibility, an ideal bone repair material, and can avert the requirement for the secondary surgical procedure of removal. In this study, nano-hydroxyapatite combined with a polyurethane containing 3% silver (Ag/n-HA/PU) was synthesized, and investigated for its efficacy of treating chronic bone infection with bone defects. To clarify its silver ions release characteristics, the concentration of the Ag+ in the elution was analyzed every day after in vitro deionized water immersion. A chronic osteomyelitis of tibia in rabbit model was established, and 70 New Zealand rabbits were divided into 4 groups, including the blank control group, nano-hydroxyapatite combined with polyurethane (nHA/PU) implant group, 3% Ag/n-HA/PU group and 10% Ag/n-HA/PU group after debridement. Routine blood tests, radiography, Micro-CT, and histological staining were conducted at 4 days, 3, 6 and 12 weeks post-treatment. The results showed that the released silver from the 3% Ag/n-HA/PU and 10% Ag/n-HA/PU exhibited an initial burst release and followed by a slow controlled release up to 39 days and 42 days respectively. A good repair of bone defects, an appropriate rate of degradation of scaffolds and no significant toxicity were observed in the 3% Ag/n-HA/PU group, indicating the advantages of this novel synthetic scaffold to be a potential option for the treatment of chronic osteomyelitis.
Graphical Abstract A novel nano-composite, nano-hydroxyapatite combined with a polyurethane containing 3% silver (Ag/n-HA/PU) provide controlled release of Ag+, illustrated by its abilities of biodegradation, antimicrobial activity, and favorable repair of bone defects in the treatment of chronic osteomyelitis.
These authors contributed equally: Dongli Zhang, Wen Liu * Wei Huang [email protected] 1
Department of orthopedic Surgery, The First Affiliated Hospital of
Chongqing Medical University, Chongqing 400016, China 2
Research Center for Nano-Biomaterials, Analytical & Testing Center, Sichuan University, Chengdu 610064, China
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1 Introduction Medical diagnosis and treatment technology has improved in recent years, however, the treatment of chronic bone infection remains an arduous issue for orthopedic doctors, which causes enormous e
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