Efficiency of Ampicillin-Associated Biogenic Ferrihydrite Nanoparticles in Combination with a Magnetic Field for Local T
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Bulletin of Experimental Biology and Medicine, Vol. 169, No. 5, September, 2020
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BIOTECHNOLOGIES Efficiency of Ampicillin-Associated Biogenic Ferrihydrite Nanoparticles in Combination with a Magnetic Field for Local Treatment of Burns E. V. Inzhevatkin1, O. A. Kolenchukova2,3, K. G. Dobretsov1, V. P. Ladygina1, A. V. Boldyreva1, and S. V. Stolyar1,3
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 169, No. 5, pp. 614-618, May, 2020 Original article submitted January 22, 2020 We studied the effectiveness of using magnetic ferrihydrite nanoparticles of bacterial origin carrying ampicillin for local treatment of burn wounds in rats using a magnetic field. It was found that the use of these nanoparticles in combination with a magnetic field accelerated wound healing and reduced the titer of microorganisms in comparison with the corresponding parameters in the untreated animals and animals treated with nanoparticles or ampicillin alone. Key Words: treatment of burns; bacteria; ferrihydrite nanoparticles; magnetic field Development of new medicinal products for targeted delivery of the active substance to the pathological focus is one of the most promising tasks of modern experimental medicine. Nanoobjects with magnetic properties are often considered as the means of targeted delivery. This requires the development of safe technologies for guiding these objects using a magnetic field. Ferrihydrite 5Fe2O3×9H2O is a candidate magnetically controlled drug carrier. Despite the fact that ferrihydrite is an antiferromagnetic, its magnetic properties cardinally change with decreasing the sample to a nanoscale size due to incomplete compensation magnetic moments of the sublattices; as a result, ferrihydrite nanoparticles (NP) acquire a constant magnetic moment [1]. A technology for the production of biogenic magnetic ferrihydrite NP from the Klebsiella oxytoca Federal Research Center Krasnoyarsk Science Center, Siberian Division of the Russian Academy of Sciences; 2Research Institute of Medical Problems of the North; 3Siberian Federal University, Krasnoyarsk, Russia. Address for correspondence: [email protected]. E. V. Inzhevatkin 1
biomass has been developed at the Krasnoyarsk Science Center. These bacteria were isolated from the sapropel of Lake Borovoye, Krasnoyarsk Kray [5-7]. Under anaerobic conditions, this bacterium produces a secretory exopolysaccharide associated with ferrihydrite NP using iron citrate as a substrate [8]. It was previously shown that these magnetic NP penetrate into tissues under the influence of an external magnetic field and then are eliminated from the body of experimental animals [2], they produce no cytotoxic effect and form stable complexes with an antibiotic [911]. This suggested the possibility of using ferrihydrite NP as a magnetically controlled antibiotic carrier to combat purulent inflammation of burn wounds. Here we studied the possibility of using a complex of ferrihydrite NP and ampicillin antibiotic for local treatment of burn wounds in rats.
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