Efficient antioxidant defence systems of spring barley in response to stress induced jointly by the cyst nematode parasi
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Efficient antioxidant defence systems of spring barley in response to stress induced jointly by the cyst nematode parasitism and cadmium exposure Mateusz Labudda & Ewa Muszyńska & Marta Gietler & Elżbieta Różańska & Anna Rybarczyk-Płońska & Justyna Fidler & Beata Prabucka & Abdelfattah A. Dababat
Received: 16 May 2020 / Accepted: 7 September 2020 # The Author(s) 2020
Abstract Aims This research aimed to establish how Hordeum vulgare responds to abiotic and biotic stress affecting in tandem. Methods Plants were inoculated with Heterodera filipjevi and treated with cadmium (Cd) concentration (5 μM) that can occur in the cultivated soil. To verify the hypothesis about participation of increased antioxidative defence in H. vulgare under stress, biochemical and microscopic methods were implemented. Results The amount of superoxide anions and hydrogen peroxide was diminished in plants that were both nematode-inoculated and cadmium-treated. Superoxide anions were rendered harmless by increased activity of superoxide dismutase, and H2O2 was scavenged via Foyer-Halliwell-Asada pathway. The unique enhanced antioxidant capacity of double stressed plants was also Responsible Editor: Antony Van der Ent. M. Labudda (*) : M. Gietler : A. Rybarczyk-Płońska : J. Fidler : B. Prabucka Department of Biochemistry and Microbiology, Institute of Biology, Warsaw University of Life Sciences-SGGW, Nowoursynowska 159, 02-776 Warsaw, Poland e-mail: [email protected] URL: https://orcid.org/0000-0001-8014-1644 E. Muszyńska : E. Różańska Department of Botany, Institute of Biology, Warsaw University of Life Sciences-SGGW, Warsaw, Poland A. A. Dababat International Maize and Wheat Improvement Center (CIMMYT), Soil Borne Pathogens Program, Ankara, Turkey
linked with the accumulation of S-nitrosoglutathione as nitrosoglutathione reductase activity was inhibited. Furthermore, stimulated activity of arginase in these plants could promote polyamine synthesis and indirectly enhance non-enzymatic antioxidant mechanism. Results indicate that different antioxidants operating together significantly restricted oxidation of lipids and proteins, thus the integrity of cell membranes and protein functions were maintained. Conclusions The ROS deactivation machinery in barley leaves showed an unusual response during stress induced by H. filipjevi infection and cadmium treatment. Plants could induce a multi-component model of stress response, to detoxify Cd ions and efficiently repair stress damage. Keywords Antioxidants . Cross-talk between Heterodera filipjevi and cadmium . Heterodera filipjevi . Hordeum vulgare . Oxidative stress . Trace metal Abbreviations APX Ascorbate peroxidase ARG Arginase ASA Reduced ascorbate CAT Catalase Chl a Chlorophyll a Chl b Chlorophyll b DHAR Dehydroascorbate reductase FW Fresh weight GOPX Guaiacol peroxidase GR Glutathione reductase GSH Reduced glutathione
Plant Soil
GSNO GSNOR GSSG POD PSII RNS ROS SA SNO SOD TBAR
S-Nitrosoglutathione Nitrosoglutathione reductase Oxidized glutathione Peroxidase P
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