Kidney Organogenesis Methods and Protocols
This book details protocols on classical methods and very modern ex vivo mammalian developmental kidney model systems. Chapters focus on generation of mosaic embryonic kidneys, culture of the kidney tissues ex vivo, embryonic kidney progenitor cell,
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Seppo Vainio Editor
Kidney Organogenesis Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Kidney Organogenesis Methods and Protocols
Edited by
Seppo Vainio Biocenter Oulu, Laboratory of Developmental Biology, Oulu University, Oulu, Finland; InfoTech Oulu, Oulu University, Oulu, Finland; Borealis Biobank of Northern Finland, Oulu University, Oulu, Finland; Faculty of Biochemistry and Molecular Medicine, Oulu University, Oulu, Finland
Editor Seppo Vainio Biocenter Oulu, Laboratory of Developmental Biology Oulu University Oulu, Finland InfoTech Oulu Oulu University Oulu, Finland Borealis Biobank of Northern Finland Oulu University Oulu, Finland Faculty of Biochemistry and Molecular Medicine Oulu University Oulu, Finland
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-9020-7 ISBN 978-1-4939-9021-4 (eBook) https://doi.org/10.1007/978-1-4939-9021-4 Library of Congress Control Number: 2018966342 © Springer Science+Business Media, LLC, part of Springer Nature 2019 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors, and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Cover Caption: Optical projection tomography-based visualization of the nephrons (in red) and the collecting duct tree (in grey). Automated spot detection function of the Imaris program was applied to highlight the nephrons (in yellow). E16.5. mouse kidney. This Humana Press imprint is published by the registered company Springer Science+Business Media, LLC, part of Springer Nature. The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface The mammalian kidney has served as a model to study the fundamental developmental mechanisms from the late 1950s when Clifford Grobstein established th
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