Organogenesis and high-frequency plant regeneration in Caryopteris terniflora Maxim. using thidiazuron
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MEDICINAL PLANTS
Organogenesis and high-frequency plant regeneration in Caryopteris terniflora Maxim. using thidiazuron Qinggui Wu 1 & Honglin Yang 1 & Yuxi Sun 1,2 & Jinyao Hu 1 & Lijuan Zou 1 Received: 27 April 2020 / Accepted: 10 August 2020 / Editor: Ted Klein # The Society for In Vitro Biology 2020
Abstract In vitro culture is employed as a means of producing plant materials from medicinal plants to meet the increasing demands of the market. Herein, we focused on an efficient protocol for organogenesis through two pathways in six explant types from Caryopteris terniflora Maxim (C. terniflora), a medicinal and ornamental plant. For direct organogenesis, the maximum number of shoots per internode explant (62.5) was achieved on Murashige and Skoog (MS) medium containing 3.0 mg L−1 thidiazuron (TDZ) and the maximum number of shoot tips per explant (45.5) was obtained by 1.5 mg L−1 TDZ, with 100% response. A protocol was also developed for indirect organogenesis from leaf, leaf-petiole, petiole, and root explants on MS basal medium containing a range of TDZ concentrations. Leaf-petiole explants induced more adventitious shoots (57.6 per explant) and leaves (31.4 per explant) than petioles (37.5 per explant) or roots (25.0 per explant), and the differentiation rate ranged from 87.2 to 100%. The regenerated shoots were elongated on MS medium containing 0.3 mg L−1α-naphthaleneacetic acid (NAA) and 1.0 mg L−1 gibberellic acid (GA3). The elongated shoots developed roots on ½ MS medium containing 0.3 mg L−1 NAA. Successful acclimatization of rooted plantlets in a greenhouse was achieved with 100% survival. Furthermore, the embryogeniclike structure was observed and the morphological characteristics of C. terniflora assessed at different stages of in vitro development. Our protocol offers, for the first time, an efficient, scalable propagation procedure for C. terniflora useful for large-scale cultivation for the purposes of conservation and genetic manipulation. Keywords Caryopteris terniflora Maxim . Organogenesis . Embryogenic-like structure . Propagation
Introduction Recently, the rapid development of traditional Chinese medicine and improved living standards have resulted in increasing demands for the use of natural herbal products. Limited availability of these plants in the wild, however, is creating a shortage crisis because of the unrestricted and unmanaged harvesting of medicinal plants and the extensive disturbance of their Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11627-020-10114-8) contains supplementary material, which is available to authorized users. * Lijuan Zou [email protected] 1
Ecological Security and Protection Key Laboratory of Sichuan Province, Mianyang Normal University, Mianyang 621000, China
2
Beijing Key Laboratory of Flavor Chemistry, Beijing Technology and Business University (BTBU), Beijing 100048, People’s Republic of China
natural habitats. In vitro culture has been employed to increase plant material availability and to thereby fac
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