Electrochemical biosensor for detection of MON89788 gene fragments with spiny trisoctahedron gold nanocrystal and target
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ORIGINAL PAPER
Electrochemical biosensor for detection of MON89788 gene fragments with spiny trisoctahedron gold nanocrystal and target DNA recycling amplification Yuanfeng Peng 1 & Ruiyi Li 2 & Minyi Yu 1 & Xiaowen Yi 1 & Haiyan Zhu 1 & Zaijun Li 1 & Yongqiang Yang 3 Received: 1 February 2020 / Accepted: 27 July 2020 # Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract The shape-controlled synthesis of gold nanocrystals via shape induction of hexadecyltrimethylammonium chloride, potassium bromide, and potassium iodide and enantioselective direction of L-cysteine is reported. The resulting gold nanocrystals (STO-Au) offer spiny trisoctahedron nanostructures with good monodispersity and enhanced exposed high-index facets and high catalytic activity. Construction of the electrochemical sensing platform for MON89788 gene involves the modification of STO-Au, thionine (Thi), and labeled bipedal DNA probe 1 or 2 (P1 or P2) for target DNA– induced recycling amplification. In the detection, two surface DNA probes were immobilized on gold electrode via the AuS bond. Then, hairpin DNA 1 (H1), Thi-STO-Au-P1, and Thi-STO-Au-P2 self-assemble into two-dimensional DNA nanopores (DNPs) on the electrode surface. Target DNA hybridizes with hairpin DNA 2 (H2) to open hairpin structure of H2. The opened H2 binds with H1 in the DNPs to release Thi-STO-Au-P1, Thi-STO-Au-P2, and target DNA by toehold-mediated strand-displacement. The utilization of target DNA–induced recycling allows one target DNA to release 2N STO-Au-labeled DNA strands, promoting significant signal amplification. The detection signal is further enhanced by the catalyzed redox reaction of Thi with STO-Au. The differential pulse voltammetric signal, best measured at − 0.18 V vs. Ag/AgCl, decreases linearly with increasing concentration of MON89788 in the range 0.02–8 × 104 fM, and the detection limit is 0.0048 fM (S/N = 3). The proposed method was successfully applied for electrochemical detection of MON89788 gene fragments in the PCR products from genetically modified soybean.
Keywords Nanomaterials . Transgenic soybean . Voltammetric method . Enzyme-free amplification . Regeneration-free biosensor
Introduction Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00604-020-04467-5) contains supplementary material, which is available to authorized users. * Zaijun Li [email protected] * Yongqiang Yang [email protected] 1
School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, China
2
Lihu Road 1800, Wuxi 214122, Jiangsu, China
3
National Graphene Product Quality Supervision and Inspection Center, Jiangsu Province Special Equipment Safety Supervision and Inspection Institute Branch, Wuxi 214071, China
DNA detection has been widely applied in disease diagnosis [1], food safety [2], biological identification [3], and archeology [4]. The main techniques for the detection of DNA are fluorescence [5], polymerase chain reaction (PCR) [6], surface-enhanced Raman spectroscopy (SERS)
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