Elucidation of Gut Microbiota in Mud Crab Scylla paramamosain Challenged to WSSV and Aeromonas hydrophila
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ORIGINAL ARTICLE
Elucidation of Gut Microbiota in Mud Crab Scylla paramamosain Challenged to WSSV and Aeromonas hydrophila Shanmeng Lin 1,2,3 & Tongtong Kong 1,2,3 & Xin Ren 1,2,3 & Shengkang Li 1,2,3 & Yi Gong 1,2,3 Received: 9 May 2020 / Accepted: 5 August 2020 # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Mud crab Scylla paramamosain (S. paramamosain) is an economically important marine crab species around the world. White spot syndrome virus (WSSV) and Aeromonas hydrophila (AH) are pathogens during mud crab mariculture. It has been reported that gut microbiota possessed a great impact on the host development, nutrition, immunity, and disease resistance. However, little information was known about the impacts of WSSV or AH infection on the structure, composition, and function of the gut microbiotain of mud crabs. In this study, the gut microbiota of mud crabs infected with A. hydrophila and WSSV were characterized. The results showed that the composition and bacteria correlation of the gut microbiota were significantly decreased. During A. hydrophila infection, the pathogens played a major regulatory role in host. While in the mud crabs infected with WSSV, many beneficial strains had a great impact on the host expect for the pathogens. Therefore, our study revealed the effect of pathogens infection on gut microbiota of mud crabs and clarified the difference between viral infection and bacterial infection. Keywords Scylla paramamosain . WSSV . Aeromonas hydrophila . Gut microbiota
Introduction It was thought that the microbiota possesses a great impact on humans and other vertebrates through affecting the health or protecting the host from diseases (Lai et al. 2009; Goffredi et al. 2013). The structure of microbiota could be impacted by chemical conditions such as temperature, moisture content,
Highlights • The gut microbiota in mud crab during A. hydrophila and WSSV infection were characterized. • The composition and bacteria correlation of the gut microbiota significantly decreased during the infections. • During A. hydrophila infection, the pathogenic bacteria play the major role in host. • During WSSV infection, many beneficial strains possess great impacts on the host expect for the pathogenic bacteria. * Yi Gong [email protected] 1
Guangdong Provincial Key Laboratory of Marine Biology, Shantou University, Shantou 515063, China
2
Institute of Marine Sciences, Shantou University, Shantou 515063, China
3
STU-UMT Joint Shellfish Research Laboratory, Shantou University, Shantou 515063, China
and physical conditions, including pH, nutrients, and oxygen (Dethlefsen et al. 2007; Dewhirst et al. 2010). ria(Hill and Artis 2010). The structure of the gut microbiota protected host against probiotics and pathogens (Buffie and Pamer 2013; Schubert et al. 2015). Moreover, many microbiota inhabiting the internal and external have been reported, and these microbiota had similar profound impacts (Shin et al. 2011). The intestine is a special tissue that has been colonized by the comp
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