Potential immune regulatory role of miR-146a upon Aeromonas hydrophila and Edwardsiella piscicida infections in zebrafis
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BACTERIAL FUNGAL AND VIRUS MOLECULAR BIOLOGY - SHORT COMMUNICATION
Potential immune regulatory role of miR-146a upon Aeromonas hydrophila and Edwardsiella piscicida infections in zebrafish T. D. Liyanage 1 & Chamilani Nikapitiya 1 & Jehee Lee 2,3 & Mahanama De Zoysa 1,3 Received: 28 October 2019 / Accepted: 30 January 2020 # Sociedade Brasileira de Microbiologia 2020
Abstract This study was aimed to understand the expression of miR-146a in zebrafish (Danio rerio) and its role in regulating immune responses during Aeromonas hydrophila and Edwardsiella piscicida infections. The miR-146a expression was observed from the 1-h post fertilization (hpf) stage and gradually increased up to the early larval stage of zebrafish. The ubiquitous expression of miR-146a was detected in all tested tissues, with the highest level in gills. The expression of miR-146a was significantly increased in larvae when exposed to E. piscicida infection at 24 and 48 h post exposure (hpe). Intraperitoneally (i.p.) injected A. hydrophila and E. piscicida into adult zebrafish showed significant upregulation of miR-146a in gills. Furthermore, immunerelated genes, toll-like receptor, tlr-4, transducing signaling pathway molecules, traf-6 and myd88 (bacteria-infected larvae and adults), transcription factor relA and mcp-1b (bacteria-infected adults), pro-inflammatory, il-6 (A. hydrophila–exposed larvae) and mmp-9 (bacteria-exposed larvae) were significantly repressed. In contrast, il-1β, tnf-α, cxcl-18b, and ccl-34a.4 were induced in both bacteria-challenged larvae and adults. Based on the results, it is suggested that endogenous miR-146a could act as an infection inducible miRNA in zebrafish upon A. hydrophila and E. piscicida infections; also, it could potentially regulate the immune responses in zebrafish. Keywords miR-146a . Zebrafish . A. hydrophila . E. piscicida
Introduction Micro RNAs (miRNAs) are small non-coding endogenous RNA, containing ~ 22 nucleotides, and they can regulate the gene expression at a post-transcriptional level by binding to 3′ untranslated regions (UTRs) of target mRNAs [1, 2]. The miRNAs play functionally important roles in a variety of Responsible Editor: Waldir P. Elias. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s42770-020-00237-w) contains supplementary material, which is available to authorized users. * Mahanama De Zoysa [email protected] 1
College of Veterinary Medicine, Chungnam National University, Yuseong-gu, Daejeon 34134, Republic of Korea
2
Department of Marine Life Sciences, Jeju National University, Jeju-siJeju Self-Governing Province, 63243, Republic of Korea
3
Fish Vaccine Research Center, Jeju National University, Jeju-siJeju Self-Governing Province, 63243, Republic of Korea
key biological processes such as cell proliferation, differentiation, apoptosis, and immunity [3, 4]. The miR-146a is one such important miRNA, which primarily contributes to regulating the inflammation, particularly with innate immune responses and evidently involves in ea
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