Enhanced production of Aspergillus niger inulinase from sugar beet molasses and its kinetic modeling
- PDF / 1,382,237 Bytes
- 17 Pages / 547.087 x 737.008 pts Page_size
- 26 Downloads / 236 Views
(0123456789().,-volV) ( 01234567 89().,-volV)
ORIGINAL RESEARCH PAPER
Enhanced production of Aspergillus niger inulinase from sugar beet molasses and its kinetic modeling Mustafa Germec . Irfan Turhan
Received: 4 March 2020 / Accepted: 13 May 2020 Ó Springer Nature B.V. 2020
Abstract Objective The fermentation medium contains many complex components (vitamins, minerals, etc.) for better growth of the microorganisms. The increasing purity and number of these components used in the medium seriously affect the cost of the microbial process. This study aimed to further optimize the concentration of the components used in the medium (yeast extract and peptone) for inulinase fabrication by Aspergillus niger from sugar-beet molasses in shake flask fermentation by using Central Composite Design (CCD) and to kinetically identify the fermentation. Results The results indicated that the optimal medium composition consisted of only 4.2% (w/v) yeast extract. By using the fermentation environment, the inulinase generation, inulinase/sucrase ratio, maximum inulinase generation rate, maximum sugar depletion rate, and substrate utilization yield were determined as 1294.5 U/mL, 1.2, 159.6 U/mL/day, 7.4 g/L/day, and 98.1%, respectively. The kinetic analysis of the fungal development (logistic model) indicated that a specific development rate and initial biomass concentration were 0.89/day and 1.79 g/L, respectively. Inulinase and sucrase productions are mixed-development associated since the a value = 0 (8.46 and 4.31 U/mgX) and the b value = 0 (5.15 and M. Germec I. Turhan (&) Department of Food Engineering, Akdeniz University, 07058 Antalya, Turkey e-mail: [email protected]
4.83 U/mgX day), respectively (Luedeking–Piret model). Besides, the maintenance value (Z) (0.009 gS/gX day) was lower than c value (1.044 gS/gX), showing that A. niger commonly uses the substrates for enzyme fabrication and fungal development (modified Luedeking–Piret model). Conclusions The enzyme activity was increased by optimizing the concentration of the components used. It was demonstrated that the proposed kinetic models can victoriously define fungal development, enzyme fabrication, and sugar depletion. Keywords Inulinase Aspergillus niger Molasses Central composite design Kinetic modeling
Introduction Enzymes moving on inulin are a substantial part of the enzymes utilized in the industrial sector within the glycoside hydrolase (GH) gene family GH32 and GH91. Both GH32 and GH91 represent thousands of GH enzymes including inulinases, invertase, 1-exohydrolase, fructan-fructosyltransferases, sucrose-fructosyltransferases, inulin lyase, and levanases. Among them, the inulinases belong to GH32. They have been widely used in the fabrication of fructose and fructooligosaccharides by targeting b-2,1 linkage in inulin. According as the regional selective reaction
123
Biotechnol Lett
and the manner of activity on inulin, they are primarily divided into two classifications: exo-inulinase (EC 3.2.1.80) and endo-inulinase (EC 3.2.1.7). Exo-i
Data Loading...
