The effect of heat stress on sugar beet recombination
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ORIGINAL ARTICLE
The effect of heat stress on sugar beet recombination Mikel Arrieta1 · Glenda Willems3 · Jérôme DePessemier3 · Isabelle Colas1 · Alexandra Burkholz3 · Aude Darracq3 · Sigrid Vanstraelen3 · Pieter Pacolet3 · Camille Barré3 · Paul Kempeneers3 · Robbie Waugh1,2 · Steve Barnes3 · Luke Ramsay1 Received: 20 May 2020 / Accepted: 9 September 2020 © The Author(s) 2020
Abstract Meiotic recombination plays a crucial role in plant breeding through the creation of new allelic combinations. Therefore, lack of recombination in some genomic regions constitutes a constraint for breeding programmes. In sugar beet, one of the major crops in Europe, recombination occurs mainly in the distal portions of the chromosomes, and so the development of simple approaches to change this pattern is of considerable interest for future breeding and genetics. In the present study, the effect of heat stress on recombination in sugar beet was studied by treating F1 plants at 28 °C/25 °C (day/night) and genotyping the progeny. F1 plants were reciprocally backcrossed allowing the study of male and female meiosis separately. Genotypic data indicated an overall increase in crossover frequency of approximately one extra crossover per meiosis, with an associated increase in pericentromeric recombination under heat treatment. Our data indicate that the changes were mainly induced by alterations in female meiosis only, showing that heterochiasmy in sugar beet is reduced under heat stress. Overall, despite the associated decrease in fertility, these data support the potential use of heat stress to foster recombination in sugar beet breeding programmes.
Introduction Sugar beet (Beta vulgaris ssp. vulgaris) is one of the major crops in Europe, providing nearly 20% of the world´s sugar production (Statistical Office of the European Communities 2018). It is a source of animal feed and is a feedstock for 30% of the bioethanol produced in Europe (SalazarOrdóñez et al. 2013). The species has an estimated genome size of around 750 Mb (Arumuganathan and Earle 1991; Dohm et al. 2014), with a diploid complement of 2n = 18 chromosomes. Communicated by Benjamin Stich. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00122-020-03683-0) contains supplementary material, which is available to authorized users. * Luke Ramsay [email protected] 1
Cell and Molecular Sciences, The James Hutton Institute, Invergowrie, Dundee DD2 5DA, UK
2
Division of Plant Sciences, University of Dundee at The James Hutton Institute, Invergowrie, Dundee DD2 5DA, UK
3
SESVanderHave, Soldatenplein 15, 3300 Tienen, Belgium
The original sugar beet breeding pool has been considered genetically narrow given its single origin in white fodder beet (Frese et al. 2009), and there has been a concerted effort in recent decades to broaden the crop’s gene pool. While the introgression of traits from wild relatives, in particular for biotic stress resistance, has been successful (Monteiro et al. 2018), it has also report
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