Enhanced resistance to fungal and bacterial diseases in tomato and Arabidopsis expressing BSR2 from rice
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ORIGINAL ARTICLE
Enhanced resistance to fungal and bacterial diseases in tomato and Arabidopsis expressing BSR2 from rice Satoru Maeda1 · Naoki Yokotani2,3 · Kenji Oda2 · Masaki Mori1 Received: 28 June 2020 / Accepted: 1 August 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Key message The overexpression of rice BSR2 would offer a simple and effective strategy to protect plants from multiple devastating diseases in tomato and Arabidopsis. Abstract Many devastating plant diseases are caused by pathogens possessing a wide host range. Fungal Botrytis cinerea and Rhizoctonia solani, as well as bacterial Pseudomonas syringae and Ralstonia pseudosolanacearum are four such pathogens that infect hundreds of plant species, including agronomically important crops, and cause serious diseases, leading to severe economic losses. However, reports of genes that can confer resistance to broad host-range pathogens via traditional breeding methods are currently limited. We previously reported that Arabidopsis plants overexpressing rice BROAD-SPECTRUM RESISTANCE2 (BSR2/CYP78A15) showed tolerance not only to bacterial P. syringae pv. tomato DC3000 but also to fungal Colletotrichum higginsianum and R. solani. Rice plants overexpressing BSR2 displayed tolerance to two R. solani anastomosis groups. In the present study, first, BSR2-overexpressing (OX) Arabidopsis plants were shown to be additionally tolerant to B. cinerea, R. solani, and R. pseudosolanacearum. Next, tomato ‘Micro-Tom’ was used as a model to determine whether such tolerance by BSR2 can be introduced into dicot crops to prevent infection from pathogens possessing wide host range. BSR2-OX tomato displayed broad-spectrum disease tolerance to fungal B. cinerea and R. solani, as well as to bacterial P. syringae and R. pseudosolanacearum. Additionally, undesirable traits such as morphological changes were not detected. Thus, BSR2 overexpression can offer a simple and effective strategy to protect crops from multiple destructive diseases. Keywords Tomato · Disease resistance · BSR2 · Botrytis cinerea · Rhizoctonia solani · Ralstonia pseudosolanacearum (syn. Ralstonia solanacearum)
Introduction
Communicated by Neal Stewart. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00299-020-02578-0) contains supplementary material, which is available to authorized users. * Masaki Mori [email protected] 1
Institute of Agrobiological Sciences, NARO (NIAS), Tsukuba, Japan
2
Research Institute for Biological Sciences, Okayama Prefectural Technology Center for Agriculture, Forestry, and Fisheries, Okayama, Japan
3
Present Address: Kazusa DNA Research Institute, Chiba, Japan
Plant diseases are a major limiting factor in crop production. To date, disease management in agriculture has relied mainly on the application of fungicides and bactericides to control pathogens; however, these pathogens generally develop resistance to such biocides. The excessive use of biocides poses risks to human heal
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