Enhancing cellulose nanofibrillation of eucalyptus Kraft pulp by combining enzymatic and mechanical pretreatments
- PDF / 2,154,058 Bytes
- 18 Pages / 547.087 x 737.008 pts Page_size
- 33 Downloads / 192 Views
(0123456789().,-volV) (0123456789().,-volV)
ORIGINAL RESEARCH
Enhancing cellulose nanofibrillation of eucalyptus Kraft pulp by combining enzymatic and mechanical pretreatments Florencia Cebreiros . Santiago Seiler . Sai Swaroop Dalli . Claudia Lareo Jack Saddler
.
Received: 21 May 2020 / Accepted: 14 October 2020 Ó Springer Nature B.V. 2020
Abstract Nanofibrillated cellulose (NFC) extracted from biomass has potential applications in material science and biomedical engineering. In this study, NFC was obtained from bleached eucalyptus Kraft pulp (BEKP) using two commercial enzyme cocktails with cellulolytic and hemicellulolytic activities and non-catalytic protein (swollenin), followed by ultrasonication. This work represents an initial study of the implementation of non-catalytic proteins along with enzymes to extract NFC from biomass. Enzymatic pretreatment was performed to partially remove hemicellulose while enhancing cellulose accessibility for NFC extraction. Cellulase pretreatment with xylanase and swollenin supplementation increased cellulose accessibility and fiber swelling due to extensive hemicellulose removal ([ 80%) and fiber F. Cebreiros C. Lareo (&) Departamento de Bioingenierı´a, Facultad de Ingenierı´a, Universidad de la Repu´blica, Julio Herrera y Reissig 565, CP 11300 Montevideo, Uruguay e-mail: [email protected] S. Seiler Norman B. Keevil Institute of Mining Engineering, Faculty of Applied Science, University of British Columbia, 517-6350 Stores Road, Vancouver, BC V6T 1Z4, Canada S. S. Dalli J. Saddler Forest Products Biotechnology/Bioenergy Group, Department of Wood Science, Faculty of Forestry, University of British Columbia, 2424 Main Mall, Vancouver, BC V6T 1Z4, Canada
morphology changes. Subsequent ultrasonication was performed for cellulose nanofibrillation resulting in high NFC yields (61–97%), while keeping NFC properties almost unchanged. Through this process, cellulose nanofibers with diameters ranging from 3 nm to 10 nm were effectively isolated from BEKP, which allows to produce high quality NFC for further applications. Keywords Nanofibrillated cellulose Enzymatic pretreatment Xylanase Swollenin Ultrasonication
Introduction Cellulose-based materials have great potential to replace petroleum-based material in technological applications due to its abundance, biodegradability, and outstanding mechanical properties. The production of cellulose nanomaterials has gained increasing attention over the past few decades due to their potential applications such as aerogel and hydrogel, reinforcement in nanocomposites, packaging materials, and biomedical materials (Abitbol et al. 2016). Two different kinds of cellulose nanomaterials can be obtained from lignocellulosic biomass based on the size, morphology and extraction method: cellulose nanocrystals or nanocrystalline cellulose (NCC) and cellulose nanofibrils or nanofibrillated cellulose (NFC). Typically, NFCs have long, entangled and
123
Cellulose
flexible fibrils with diameters and lengths ranging from 1 to 100 nm to
Data Loading...
