Eosin Y as a high-efficient photooxidase mimic for colorimetric detection of sodium azide
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RESEARCH PAPER
Eosin Y as a high-efficient photooxidase mimic for colorimetric detection of sodium azide Junren Wang 1 & Haili Yu 1 & Yi He 1 Received: 22 May 2020 / Revised: 12 August 2020 / Accepted: 18 August 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract The reported fluorescent dye–based artificial light–responsive oxidase mimics are suffering from their low catalytic efficiency. To overcome the limitation, we report the photooxidase-mimicking activity of Eosin Y which can catalyze the oxidation of various chromogenic substrates such as 3,3′,5,5′-tetramethylbenzydine (TMB), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 3,3′-diaminobenzidine (DAB), and o-phenylenediamine (OPD) by dissolved oxygen. The photooxidase-like activity of Eosin Y is highly efficient for TMB substrate, and its catalytic efficiency is higher than that of the reported fluorescein (130 fold) and 9-mesityl-10-methylacridinium ion (7.7-fold) mimetic photooxidase. Moreover, the photosensitized Eosin Y-TMB chromogenic system is utilized for colorimetric detection of highly toxic and explosive sodium azide (NaN3) in a linear range from 5 to 500 μM with a limit of detection of 3.5 μM. The resulting colorimetric assay is selective and applied to determine NaN3 in real lake water samples. Keywords Enzyme mimics . Photosensitization . Colorimetry . Sodium azide . High efficiency
Introduction The good catalytic property of natural enzymes has motivated people to develop artificial enzyme mimics to conquer their intrinsic bad stability and high cost [1–3]. To date, peroxidaseor oxidase-like activities have been demonstrated on various materials, including nanomaterials, metal-organic frameworks, metal complexes, and small organic molecules [4–7]. These artificial enzyme mimics have been successfully used for chemical sensing, drug delivery, imaging, and diagnosis because of their distinct advantages such as high stability and large-scale production [8–10]. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00216-020-02895-z) contains supplementary material, which is available to authorized users. * Haili Yu [email protected] * Yi He [email protected] 1
National Collaborative Innovation Center for Nuclear Waste and Environmental Safety, School of National Defence Science & Technology, Southwest University of Science and Technology, Mianyang 621010, China
In order to better imitate the peroxidase- or oxidase-like characteristics, several protocols have been developed to regulate their catalytic activity, such as size, surface charge, composition, morphology, and ligands of materials as well as use of external stimuli [11–15]. Among these strategies, the external light stimuli are a promising avenue to modulate the peroxidase or oxidase mimics owing to its efficiency, specificity, simplicity, and environmental friendliness. Besides, to avoid the use of unstable H2O2, photoresponsive oxidase mimics are developed, in which the dissolved oxygen is
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