Establishment and characterization of a cell line from Ghanaian Aedes aegypti (Diptera: Culicidae) focusing on Aedes -bo
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Establishment and characterization of a cell line from Ghanaian Aedes aegypti (Diptera: Culicidae) focusing on Aedes-borne flavivirus susceptibility Michael Amoa-Bosompem 1,2,3 & Daisuke Kobayashi 1 & Kentaro Itokawa 4 & Astri Nur Faizah 1,5 & Ryusei Kuwata 6 & Samuel Dadzie 3 & Takaya Hayashi 7 & Shoji Yamaoka 7 & Kyoko Sawabe 1 & Shiroh Iwanaga 2 & Haruhiko Isawa 1 Received: 1 July 2020 / Accepted: 2 September 2020 / Editor: Tetsuji Okamoto # The Society for In Vitro Biology 2020
Abstract Mosquitoes are generally considered one of the most important vectors of arboviruses, with Aedes aegypti regarded as the most important in transmission of yellow fever and dengue viruses. To investigate why there are differences in the incidence of dengue fever and Zika in different geographical areas and an absence of outbreaks in Ghana in spite of an abundance of A. aegypti mosquitoes, we established a continuous cell line from embryonic cells of A. aegypti collected in Ghana and assessed its susceptibility to dengue, yellow fever, and Zika viruses. The new cell line (designated AeAe-GH98), having an adhesive spindle-shaped web-like morphology, was serially subcultured in both VP-12 and Schneider’s medium supplemented with 10% heat-inactivated fetal bovine serum. AeAe-GH98 cells were found to have a population doubling time of 1.3 d during exponential growth. The mosquito colony used to establish the cell line was confirmed to have originated from Africa using microsatellite assay. In terms of susceptibility to Aedes-borne flaviviruses, AeAe-GH98 cells were found to have different degrees of susceptibility to yellow fever, Zika, and dengue virus infection and propagation. While susceptibility of AeAe-GH98 cells to yellow fever and Zika viruses was comparable with that of C6/36 cells, susceptibility to dengue virus was significantly lower. This cell line will serve as a useful tool for determining molecular factors influencing virus–vector susceptibility in vitro. Keywords Dengue virus . Yellow fever virus . Zika virus . Aedes aegypti . Mosquito cell lines Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11626-020-00504-y) contains supplementary material, which is available to authorized users. * Haruhiko Isawa [email protected] 1
Department of Medical Entomology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan
2
Department of Environmental Parasitology, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-850, Japan
3
Department of Parasitology, Noguchi Memorial Institute for Medical Research, University of Ghana, College of Health Sciences, P.O.Box LG581, Legon, Accra, Ghana
4
Pathogen Genomics Center, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan
5
Graduate School of Agricultural and Life Science, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
6
Faculty of Veterinary Medicine, Okayama University of Science, 1-3 Ikoinooka, Imabari, Ehi
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