Eukaryotic protein production in designed storage organelles
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BioMed Central
Open Access
Research article
Eukaryotic protein production in designed storage organelles Margarita Torrent†1, Blanca Llompart†2, Sabine Lasserre-Ramassamy1, Immaculada Llop-Tous1, Miriam Bastida2, Pau Marzabal2, Ann WesterholmParvinen3, Markku Saloheimo3, Peter B Heifetz*2 and M Dolors Ludevid*1 Address: 1Departament de Genètica Molecular, Consorci CSIC-IRTA, Jordi Girona, 08034 Barcelona, Spain, 2ERA Biotech, S.A. Parc Científic de Barcelona, Baldiri Reixac, 08028 Barcelona, Spain and 3VTT Technical Research Centre, PO Box 1000, FIN-02044VTT, Finland Email: Margarita Torrent - [email protected]; Blanca Llompart - [email protected]; Sabine LasserreRamassamy - [email protected]; Immaculada Llop-Tous - [email protected]; Miriam Bastida - [email protected]; Pau Marzabal - [email protected]; Ann Westerholm-Parvinen - [email protected]; Markku Saloheimo - [email protected]; Peter B Heifetz* - [email protected]; M Dolors Ludevid* - [email protected] * Corresponding authors †Equal contributors
Published: 28 January 2009 BMC Biology 2009, 7:5
doi:10.1186/1741-7007-7-5
Received: 13 January 2009 Accepted: 28 January 2009
This article is available from: http://www.biomedcentral.com/1741-7007/7/5 © 2009 Torrent et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract Background: Protein bodies (PBs) are natural endoplasmic reticulum (ER) or vacuole plantderived organelles that stably accumulate large amounts of storage proteins in seeds. The prolinerich N-terminal domain derived from the maize storage protein γ zein (Zera) is sufficient to induce PBs in non-seed tissues of Arabidopsis and tobacco. This Zera property opens up new routes for high-level accumulation of recombinant proteins by fusion of Zera with proteins of interest. In this work we extend the advantageous properties of plant seed PBs to recombinant protein production in useful non-plant eukaryotic hosts including cultured fungal, mammalian and insect cells. Results: Various Zera fusions with fluorescent and therapeutic proteins accumulate in induced PBlike organelles in all eukaryotic systems tested: tobacco leaves, Trichoderma reesei, several mammalian cultured cells and Sf9 insect cells. This accumulation in membranous organelles insulates both recombinant protein and host from undesirable activities of either. Recombinant protein encapsulation in these PBs facilitates stable accumulation of proteins in a protected subcellular compartment which results in an enhancement of protein production without affecting the viability and development of stably transformed hosts. The induced PBs also retain the high-density properties of native seed PBs which facilitate the recovery and purification of the recombinant proteins they conta
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