Cell-Free Protein Production Methods and Protocols
During the past decade as the data on gene sequences and expression patterns rapidly accumulated, cell-free protein synthesis technology has also experienced a revolution, becoming a powerful tool for the preparation of proteins for their functional and s
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Molecular Biology™
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For other titles published in this series, go to www.springer.com/series/7651
Cell-Free Protein Production Methods and Protocols
Edited by
Yaeta Endo Cell-Free Science and Technology Research Center, Ehime University, Ehime, Japan
Kazuyuki Takai Cell-Free Science and Technology Research Center, Ehime University, Ehime, Japan and
Takuya Ueda The Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Chiba, Japan
Editors Yaeta Endo Cell-Free Science and Technology Research Center Ehime University Ehime Japan [email protected]
Kazuyuki Takai Cell-Free Science and Technology Research Center Ehime University Ehime Japan [email protected]
Takuya Ueda The Department of Medical Genome Sciences Graduate School of Frontier Sciences The University of Tokyo Kashiwa, Chiba Japan [email protected]
ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-60327-330-5 e-ISBN 978-1-60327-331-2 DOI 10.1007/978-1-60327-331-2 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2009933999 © Humana Press, a part of Springer Science+Business Media, LLC 2010 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. While the advice and information in this book are believed to be true and accurate at the date of going to press, neither the authors nor the editors nor the publisher can accept any legal responsibility for any errors or omissions that may be made. The publisher makes no warranty, express or implied, with respect to the material contained herein. Printed on acid-free paper Humana Press is part of Springer Science+Business Media (www.springer.com)
Preface Understanding how living organisms handle and transfer their genetic information at the molecular level is the major purpose of molecular biology. This, of course, includes the understanding of proteins that constitute the major part of the activity of living cells. However, the analysis of protein molecules has been running behind that of nucleic acids. This is clear because proteins are much more difficult to handle than nucleic acids. We have experienced an unprecedented decade during which the data on gene sequences and expression patterns has accumulat
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