Evaluation of fish skin as a biological dressing for metacarpal wounds in donkeys
- PDF / 5,366,366 Bytes
- 10 Pages / 595.276 x 790.866 pts Page_size
- 55 Downloads / 195 Views
RESEARCH ARTICLE
Open Access
Evaluation of fish skin as a biological dressing for metacarpal wounds in donkeys Ahmed Ibrahim1*† , Mahmoud Soliman2†, Saber Kotb3 and Magda M. Ali4
Abstract Background: The use of biological dressings has recently emerged in the management of burns and wounds. The aim of the present study was to evaluate the Nile tilapia skin as a biological dressing for full-thickness cutaneous metacarpal wounds in donkeys. The study was conducted on nine clinically healthy donkeys (n = 9). Here, fish skin dressings were obtained from fresh Nile tilapia (Oreochromis niloticus and sterilized by immersion in silver nanoparticles (AgNPs) solution for 5 min, with no change in collagen content. Bilateral, circular full-thickness excisional skin wounds (2 cm in diameter) were created on the dorsal aspect of the mid-metacarpals of each donkey. Wounds on the right metacarpals (treated wounds, n = 9) were dressed with sterile fish skins, while wounds on the left metacarpals (control wounds, n = 9) were dressed with sterile non-adherent dressing pads without any topical applications. Wound dressings were changed weekly. Wounds were evaluated microbiologically, grossly, and histologically on days 7, 14, and 21 post-wound inductions. Results: Fish skin-dressed wounds showed a significant (P < 0.0001) reduction in microbial counts (Total viable bacterial count, Staphylococcal count, and Coliform count), a significant (P < 0.0001) decrease in the wound size, and a significant reduction (P < 0.0001) in the epithelial gap compared to the untreated wounds. No frequent dressing changes were needed. Conclusions: Fish skin dressing accelerated the wound healing process and efficiently inhibited the local microbial activity and exuberant granulation tissue formation suggesting its reliable and promising application for metacarpal wounds of donkeys. Keywords: Fish skin, Tilapia, Silver nanoparticles, Dressing, Wounds, Donkeys
Background Wounds of the distal limbs of equines are common and represent more than 60% of all wounds. These wounds are more problematic than others located elsewhere in the body due to its high susceptibility to contamination, the poor healing nature, and high tendency to form exuberant granulation tissue [1]. Biological wound dressings are that derived from natural resources and are known for their biocompatibility * Correspondence: [email protected]; [email protected] † Ahmed Ibrahim and Mahmoud Soliman contributed equally to this work. 1 Veterinary Teaching Hospital, Faculty of Veterinary Medicine, Assiut University, Assiut 71526, Egypt Full list of author information is available at the end of the article
and biodegradability [2]. Several biological materials have been used for wound dressing in equines including: the porous bovine collagen membrane, keratinocyte collagen membrane, split-thickness allogeneic skin, allogeneic peritoneum, and xenogeneic porcine small intestinal submucosa [3–5]. Fish skin contains collagen type I and III in large quantities [6], a protein that is potenti
Data Loading...
