Evaluation of pharmacological potentials of the aerial part of Achyranthes aspera L.: in vivo, in vitro and in silico ap
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RESEARCH ARTICLE
Evaluation of pharmacological potentials of the aerial part of Achyranthes aspera L.: in vivo, in vitro and in silico approaches Nazim Uddin Emon1 · Safaet Alam2 · Sajib Rudra3 · Sabiha Chowdhury4 · Joy Chandra Rajbangshi5 · Amlan Ganguly4 Received: 21 September 2020 / Accepted: 1 November 2020 © Institute of Korean Medicine, Kyung Hee University 2020
Abstract Achyranthes aspera L. (Prickly Chaff flower) is an imperative Asian herb that has been known for numerous healing powers. The aim of this research is to investigate the pharmacological i.e., antidepressant and anxiolytic properties of the methanol extract of aerial part of A. aspera (MEAA) by in vivo and in silico approaches. Additionally, thrombolytic potential of the crude extract has also been explored. The antidepressant and anxiolytic investigations were performed by force swimming test (FST), tail suspension test (TST), and hole board test (HBT) in mice, whereas clot lysis activity test was conducted using thrombolytic experimental method. Furthermore, in silico studies were appraised with Discovery studio, PyRx, UCSF Chimera whereas, PASS prediction, and ADME/T was implemented using online tools. Treatment with MEAA revealed significant (p < 0.05) reduction of immobility time in FST and TST, increased head dipping and decreased the latency of time in HBT. Besides, MEAA produced significant (p < 0.001) clot lysis activity at a minimum dose. In the computer-aided approach, among all the proteins, the docking score has been revealed ranging from − 2.31 kcal/mol to − 7.4 kcal/mol. Spathulenol, diazepam and hydroquinone showed the best binding affinity and all compounds were found safe in ADME/T study. The outcomes of this research demonstrate that MEAA may provide potential for mitigating depression, anxiety, and coagulation. Keywords Achyranthes aspera L. · Antidepressant · Anxiolytic · Thrombolytic · In silico
Nazim Uddin Emon and Safaet Alam have contributed equally to this work. * Safaet Alam [email protected] * Amlan Ganguly [email protected] 1
Department of Public Health, School of Science and Technology, BOU, Gazipur, Dhaka 1705, Bangladesh
2
Department of Pharmacy, State University of Bangladesh, 77 Satmasjid Road, Dhanmondi, Dhaka 1207, Bangladesh
3
Department of Botany, Faculty of Biological Science, University of Chittagong, Chittagong 4331, Bangladesh
4
Department of Clinical Pharmacy and Pharmacology, Faculty of Pharmacy, University of Dhaka, Dhaka 1000, Bangladesh
5
Department of Pharmacy, Comilla University, Kotbari, Cumilla 3506, Bangladesh
Abbreviations ADME/T Absorption, distribution, metabolism, excretion, and toxicity BDNF Brain-derived neurotrophic factor CADD Computer aided drug discovery FST Force swimming test GABA Gamma-aminobutyric acid HBT Hole board test MEAA Methanol extract of aerial part of A. asperaMethanol extract of aerial part of A. aspera NT-3 Neurotrophin-3 PASSPASS Predictions of activity of substances spectra SKSK Streptokinase SSRI Selective serotonin re
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