Exploring Lassa Virus Proteome to Design a Multi-epitope Vaccine Through Immunoinformatics and Immune Simulation Analyse
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Exploring Lassa Virus Proteome to Design a Multi‑epitope Vaccine Through Immunoinformatics and Immune Simulation Analyses Sifat Bin Sayed1 · Zulkar Nain1 · Md. Shakil Ahmed Khan1 · Faruq Abdulla2 · Rubaia Tasmin3 · Utpal Kumar Adhikari4 Accepted: 22 December 2019 © Springer Nature B.V. 2020
Abstract Lassa virus (LASV) is responsible for a type of acute viral haemorrhagic fever referred to as Lassa fever. Lack of adequate treatment and preventive measures against LASV resulted in a high mortality rate in its endemic regions. In this study, a multi-epitope vaccine was designed using immunoinformatics as a prophylactic agent against the virus. Following a rigorous assessment, the vaccine was built using T-cell (NCTL = 8 and NHTL = 6) and B-cell (NLBL = 4) epitopes from each LASV-derived protein in addition with suitable linkers and adjuvant. The physicochemistry, immunogenic potency and safeness of the designed vaccine (~ 68 kDa) were assessed. In addition, chosen CTL and HTL epitopes of our vaccine showed 97.37% worldwide population coverage. Besides, disulphide engineering also improved the stability of the chimeric vaccine. Molecular docking of our vaccine protein with toll-like receptor 2 (TLR2) showed binding efficiency followed by dynamics simulation for stable interaction. Furthermore, higher levels of cell-mediated immunity and rapid antigen clearance were suggested by immune simulation and repeated-exposure simulation, respectively. Finally, the optimized codons were used in in silico cloning to ensure higher expression within E. coli K12 bacterium. With further assessment both in vitro and in vivo, we believe that our proposed peptide-vaccine would be potential immunogen against Lassa fever. Keywords Lassa fever · Immunoinformatics · Peptide vaccine · Molecular docking · Immune simulation
Introduction Lassa virus (LASV) is an emerging viral pathogen belongs to the Arenaviridae family and can cause severe viral haemorrhagic fever which is known as Lassa fever, with a 20% fatality rate (Charrel and De Lamballerie 2003). Lassa fever Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10989-019-10003-8) contains supplementary material, which is available to authorized users. * Utpal Kumar Adhikari [email protected] 1
Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Islamic University, Kushtia 7003, Bangladesh
2
Department of Statistics, Faculty of Sciences, Islamic University, Kushtia 7003, Bangladesh
3
Department of Pharmacy, Faculty of Biological Sciences, Islamic University, Kushtia 7003, Bangladesh
4
School of Medicine, Western Sydney University, Campbelltown, NSW 2560, Australia
is a viral acute zoonotic disease due to its capability to affect the highest number of people (~ 500,000) and causes 5000 annual deaths in Western Africa (Ogbu et al. 2007). Also, the people of Ghana (~ 10%), Côte d’Ivoire (~ 30), Nigeria (~ 40%), Guinea (~ 50%), Sierra- Leone and Liberia (~ 80%) and a few ar
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