Fabrication and Characterization of Electrospun Scaffold Based on Polycaprolactone-Aloe vera and Polyvinyl Alcohol for S
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ISSN 1229-9197 (print version) ISSN 1875-0052 (electronic version)
Fabrication and Characterization of Electrospun Scaffold Based on Polycaprolactone-Aloe vera and Polyvinyl Alcohol for Skin Tissue Engineering Marjan Shabannejad1, Mohammad Sadegh Nourbakhsh1,2*, Amir Salati3, and Zohreh Bahrami4 1
Department of Biomedical Engineering, Faculty of New Sciences and Technologies, Semnan University, Semnan 3513119111, Iran 2 Faculty of Materials and Metallurgical Engineering, Semnan University, Semnan 3513119111, Iran 3 Nervous System Stem Cells Research Center, Semnan University of Medical Sciences, Semnan 3514799442, Iran 4 Nanochemistry Group, Faculty of New Sciences and Technologies, Semnan University, Semnan 3513119111, Iran (Received September 5, 2019; Revised November 30, 2019; Accepted December 6, 2019) Abstract: In the present work, the biodegradable nanofibrous scaffolds containing poly(caprolactone) (PCL), poly(vinyl alcohol) (PVA) and aloe vera (AV), through two nozzles electrospinning method, were prepared and characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, contact angle measurement and mechanical analysis. The biocompatibility and cell growth were evaluated using MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) assay. Adding poly(vinyl alcohol) (PVA) and poly(caprolactone) (PCL) boosted the electrospinability of aloe vera (AV) solution and mechanical features of the scaffolds. According to the Scanning electron microscopy (SEM) results, uniform PVA/AV-PCL nanofibers were fabricated with the average diameter about 119±11.78 nm. Fourier-transform infrared spectroscopy) FTIR (confirmed the presence of functional groups of scaffolds. The results of the contact angle showed that by adding aloe vera, the hydrophilicity increased. The results showed that the tensile strength of samples with 6 mg/ml AV, reached to 2.02 MPa. MTT results showed that all samples had a cell viability of over 80 %. It can be concluded that nanofibrous scaffolds with concentration of 6 mg/ml, distance between syringe tip/collector of 15 cm, feed rate of 3 ml/h and applied voltage of 12 kV, have appropriate properties for skin tissue engineering by stimulating the fibroblast cells and therefore accelerating the regeneration processes of damaged skin. Keywords: Electrospinning, Aloe vera, Poly(caprolactone), Poly(vinyl alcohol), Tissue engineering
the collector) and environmental factors (humidity and pressure) [8-16]. Nanofibers have been produced by applying various natural and synthetic polymers. Polycaprolactone is a semi-crystalline, hydrophobic, and non-toxic polymer with excellent mechanical properties, good biocompatibility, low antigenicity, simple processability, and low melting point. Owing to its flexible structure, polycaprolactone can be used to improve the elasticity of rubbers. It can be also employed for medical purposes due to its biocompatibility and low biodegradability [17]. Poly(vinyl alcohol) is a biodegradable water-soluble synthetic polymer possessing
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