Fabrication of Microfluidic Cell Culture Platform for Real-time Monitoring of Lidocaine Concentration
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International Journal of Precision Engineering and Manufacturing https://doi.org/10.1007/s12541-020-00429-2
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Fabrication of Microfluidic Cell Culture Platform for Real‑time Monitoring of Lidocaine Concentration Hye Jin Choi1 · Tran Bao Ngoc1 · Hyunjik Song2 · Chul Ho Chang2 · Gyu Man Kim1 Received: 29 May 2020 / Revised: 11 September 2020 / Accepted: 14 October 2020 © Korean Society for Precision Engineering 2020
Abstract We describe a microfluidic cell culture platform for real-time measurement of drug concentrations during cell cultivation. This is the first attempt to quantify drug concentrations during cell cultivation in real-time combining ultraviolet–visible (UV–Vis) spectroscopy with a microfluidic cell culture system in a single microfluidic chip. The microfluidic chip has two specific micro-chambers, one for spectroscopic measurements and the other for cell culture. NIH/3T3 fibroblast cells were seeded and cultured in the microfluidic chip, and then the local anesthetic lidocaine was applied and its concentration was monitored in real time. The test results showed that lidocaine negatively affected the cell growth. The lidocaine concentration was decreased due to its intake by cells for the first 3 h, after which an equilibrium concentration was reached. This is a simple but innovative method that can observe and analyze the correlation between cells and drugs in real time. The microfluidic system presented here can be a useful tool in various fields such as in cytotoxicity and drug screening studies. Keywords Microfluidic cell culture platform · Continuous monitoring · Lidocaine · UV-Vis spectrometry
1 Introduction Over the last few years, research on cell using a microfluidic device as a cell culture platform has been increased in popularity. The use of a microfluidic platform has allowed the minimized use of cells and culture media owing to the Chul Ho Chang and Gyu Man Kim contributed equally to this work as corresponding author. * Chul Ho Chang [email protected] * Gyu Man Kim [email protected] Hye Jin Choi [email protected] Tran Bao Ngoc [email protected] Hyunjik Song [email protected] 1
School of Mechanical Engineering, Kyungpook National University, 80 Daehakro, Buk‑gu, Daegu 41566, Korea
Department of Anesthesiology and Pain Medicine and Anesthesia and Pain Research Institute, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul, Korea
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miniaturized size of the microfluidic device. Furthermore, as they are cultured in a microfluidic device, the status and behavior of the cells could be observed in real time by optical or confocal fluorescence microscopy [1–9]. This microscopic observation could be achieved either by placing a microscope inside the cell culture chamber [7], or by preparing a miniaturized culture chamber on the microscopy system [8, 9]. These real-time optical monitoring systems aimed to hurdle the drawback of conventional cell culture in which cell culture is interrupted by taking cells out from incubator for optical obs
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