Fine mapping of leaf rust resistance gene Rph13 from wild barley
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ORIGINAL ARTICLE
Fine mapping of leaf rust resistance gene Rph13 from wild barley Matthias Jost1 · Davinder Singh2 · Evans Lagudah1 · Robert F. Park2 · Peter Dracatos2 Received: 25 September 2019 / Accepted: 14 February 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Key message Fine mapping of the barley leaf rust resistance locus Rph13 on chromosome 3HL facilitates its use in breeding programs through marker-assisted selection. Abstract Barley leaf rust (BLR—caused by Puccinia hordei) is a widespread fungal disease that can be effectively controlled by genetic resistance. There is an ongoing need to both diversify and genetically characterise resistance loci to provide effective and durable control given the ongoing threat of rapidly evolving P. hordei populations. Here, we report on the molecular genetic characterisation of the Rph13 locus, originally derived from wild barley and transferred to barley accession Berac (then referred to as PI 531849). The 2017 reference genome of cv. Morex was used as a road map to rapidly narrow both a genetic and physical intervals around the Rph13 resistance locus. Using recombination-based mapping, we narrowed the physical interval to 116.6 kb on chromosome 3H in a segregating population of a cross of the Rph13 carrying resistant line PI 531849 with the leaf rust-susceptible cultivar Gus. We identified two nucleotide-binding leucine-rich repeat genes as likely candidates for the Rph13 resistance. Sequences from the candidate genes enabled the development of a KASP marker that distinguished resistant and susceptible progeny and was found to be predictive and useful for MAS.
Introduction Barley is the fourth most important cereal crop in global production. Barley production is, however, affected by numerous diseases that reduce both yield and grain quality. Spreading from host to host by wind- or human-aided spore migration, rust pathogens (Puccinia spp.) cause some of the most damaging diseases, especially in cereals including: wheat, barley and oat. Almost all of Australia’s devastating rust epidemics have followed unpredictable exotic rust incursions. Barley leaf rust (BLR) is a disease caused by the basidiomycete fungal pathogen Puccinia hordei Otth., which has been reported to significantly reduce grain quality and Communicated by Albrecht E. Melchinger. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00122-020-03564-6) contains supplementary material, which is available to authorized users. * Peter Dracatos [email protected] 1
CSIRO Agriculture and Food, Commonwealth Scientific and Industrial Research Organisation, GPO Box 1700, Canberra, ACT 2601, Australia
Plant Breeding Institute, The University of Sydney, Cobbitty, NSW 2570, Australia
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yield (up to 62%) given suitable environmental conditions (Cotterill et al. 1992). The fungus mostly propagates via asexual modes of reproduction, but in the presence of the alternate host (Ornithogalum spp.) can also undergo sexual recombin
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