A novel leaf rust resistance gene introgressed from Aegilops markgrafii maps on chromosome arm 2AS of wheat
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ORIGINAL ARTICLE
A novel leaf rust resistance gene introgressed from Aegilops markgrafii maps on chromosome arm 2AS of wheat K. Rani1,3 · B. R. Raghu1,4 · S. K. Jha1 · Priyanka Agarwal1 · Niharika Mallick1 · M. Niranjana1 · J. B. Sharma1 · A. K. Singh2 · N. K. Sharma1 · S. Rajkumar2 · S. M. S. Tomar1 · Vinod1 Received: 17 October 2019 / Accepted: 25 May 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Key message A novel leaf rust resistance gene, LrM, introgressed from Aegilops markgrafii and mapped on chromosome 2AS using SSR- and SNP-based PCR markers will aid in broadening the genetic base of rust resistance in wheat. Abstract A new leaf rust resistance gene tentatively named LrM was introgressed from the diploid non-progenitor species Ae. markgrafii (2n = 2x = 14, genome CC) into common wheat using the nulli-5B mechanism. The introgression line ER9700 showed a high degree of resistance against a wide spectrum of Puccinia triticina pathotypes. Genetic analysis was performed using the F1, F2, F2:3 and BC1F1 generations derived from the cross ER9-700/Agra Local. The results showed a single dominant gene for leaf rust resistance. The resistance gene LrM was mapped on chromosome arm 2AS using SSRand SNP-based PCR markers. Preliminary mapping with SSR markers in the F 2:3 population from the cross ER9-700/Agra Local identified two SSR markers flanking the LrM. SNPs were identified in the genomic region flanked by SSR markers, and SNP-based PCR markers were developed to construct the final map. Three SNP-based PCR markers co-segregated and mapped closest to the resistance gene at a distance of 2 cM. The gene LrM was distinguished from all the other genes designated and mapped on chromosome arm 2AS by molecular markers and rust reaction. All five markers used in the mapping amplified identical alleles in the donor Ae. markgrafii accession and introgression line ER9-700. The chromosomal location and rust reaction suggest that LrM is a novel leaf rust resistance gene that may be useful in broadening the genetic base of leaf rust resistance in wheat.
Introduction Communicated by Steven S. Xu. K. Rani, B. R. Raghu and S. K. Jha have contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00122-020-03625-w) contains supplementary material, which is available to authorized users. * Vinod [email protected] 1
Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India
2
ICAR-National Bureau of Plant Genetic Resources, New Delhi 110012, India
3
ICAR-Directorate of Groundnut Research, Junagadh 362001, India
4
ICAR-Indian Institute of Horticultural Research, Bengaluru 560089, India
Leaf rust caused by Puccinia triticina Eriks. is one of the major fungal diseases of wheat (Triticum aestivum L.) and inflicts substantial yield losses worldwide (Roelfs et al. 1992; Marasas et al. 2004; Kolmer 2005; Tomar et al. 2014). Among the three rusts that occur on wheat, le
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