High-resolution mapping of Rym14 Hb , a wild relative resistance gene to barley yellow mosaic disease
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ORIGINAL ARTICLE
High‑resolution mapping of Rym14Hb, a wild relative resistance gene to barley yellow mosaic disease Hélène Pidon1 · Neele Wendler2 · Antje Habekuβ3 · Anja Maasberg4 · Brigitte Ruge‑Wehling5 · Dragan Perovic3 · Frank Ordon3 · Nils Stein1,6 Received: 6 August 2020 / Accepted: 18 November 2020 © The Author(s) 2020
Abstract Key message We mapped the Rym14Hb resistance locus to barley yellow mosaic disease in a 2Mbp interval. The cosegregating markers will be instrumental for marker-assisted selection in barley breeding. Abstract Barley yellow mosaic disease is caused by Barley yellow mosaic virus and Barley mild mosaic virus and leads to severe yield losses in barley (Hordeum vulgare) in Central Europe and East-Asia. Several resistance loci are used in barley breeding. However, cases of resistance-breaking viral strains are known, raising concerns about the durability of those genes. Rym14Hb is a dominant major resistance gene on chromosome 6HS, originating from barley’s secondary genepool wild relative Hordeum bulbosum. As such, the resistance mechanism may represent a case of non-host resistance, which could enhance its durability. A susceptible barley variety and a resistant H. bulbosum introgression line were crossed to produce a large F2 mapping population (n = 7500), to compensate for a ten-fold reduction in recombination rate compared to intraspecific barley crosses. After high-throughput genotyping, the Rym14Hb locus was assigned to a 2Mbp telomeric interval on chromosome 6HS. The co-segregating markers developed in this study can be used for marker-assisted introgression of this locus into barley elite germplasm with a minimum of linkage drag.
Introduction Viruses are an increasing threat to crops worldwide. The soil-borne barley yellow mosaic disease, caused by a complex of two Bymoviruses (Barley yellow mosaic virus (BaYMV) and Barley mild mosaic virus (BaMMV)) is one Communicated by Kevin Smith. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00122-020-03733-7) contains supplementary material, which is available to authorized users.
of the most important diseases of winter barley. Widespread in central Europe and East-Asia, it causes severe yield losses up to even total crop failure (Plumb et al. 1986; Jianping 2005; Kühne 2009). As chemical control of those viruses, transmitted by the plasmodiophorid Polymyxa graminis (Kanyuka et al. 2003), is not possible, only the use of resistant varieties can preserve yield in infected fields. To date, 20 barley resistance genes have been identified, almost exclusively conferring recessive resistance (Jiang et al. 2020). Two of these loci have been cloned: the EUKARYOTIC TRANSLATION INITIATION FACTOR
* Hélène Pidon pidon@ipk‑gatersleben.de
4
KWS LOCHOW GMBH, Ferdinand‑von‑Lochow‑Straße 5, 29303 Bergen, Germany
* Nils Stein stein@ipk‑gatersleben.de
5
Institute for Breeding Research On Agricultural Crops, Julius Kühn Institute (JKI), Groß Lüsewitz, Rudolf‑Schick‑Platz 3a, 1819
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