First report of Ageratum latent virus in Veronica species and in New Zealand
- PDF / 224,412 Bytes
- 3 Pages / 595.276 x 790.866 pts Page_size
- 100 Downloads / 167 Views
First report of Ageratum latent virus in Veronica species and in New Zealand Anastasija Podolyan 1
& Arnaud G. Blouin
2
& Manpreet K. Dhami
1
& Stella Veerakone
3
& Robin MacDiarmid
2,4
Received: 29 July 2020 / Accepted: 22 October 2020 # Australasian Plant Pathology Society Inc. 2020
Abstract A new strain of Ageratum latent virus (AGLV) was found in symptomless leaves of Veronica bollonsii, V. breviracemosa and V. dieffenbachii - the native shrubs in New Zealand. The partial genome sequence of the AGLV from V. bollonsii has 85.9– 89.1% amino acid similarity to the Australian isolate AGLV-1998. This is the first record of the AGLV in Veronica species and in New Zealand. Keywords dsRNA-specific antibody . MiSeq . 5’ RACE . Indigenous
Ageratum latent virus (AGLV) (Genus Ilarvirus, family Bromoviridae) has a tripartite, positive-strand RNA genome which encodes five proteins: the replication protein 1a on the RNA1, the RNA-dependent RNA polymerase (RdRp), the protein 2b on the RNA2, and the movement (MP) and coat (CP) proteins on the RNA3 (Bujarski et al. 2019). Previously known as a strain of tobacco streak virus (TSV-1998), Ageratum latent virus was shown to be a separate species in 2013 (Sharman and Thomas 2013). AGLV has been previously reported from plants of Ageratum houstonianum and Gomphocarpus physocarpus in eastern Australia (Sharman and Thomas 2013). Infected hosts were typically symptomless, and this virus has been noted for transmission via pollen and thrips (Bujarski et al. 2019). Advanced sequencing methods have enabled the discovery of many new viruses in asymptomatic wild plants (Roossinck et al. 2015). In New Zealand, despite concerted efforts (Blouin
* Anastasija Podolyan [email protected] 1
Biocontrol and Molecular Ecology, Manaaki Whenua – Landcare Research, Lincoln 7640, New Zealand
2
New Zealand Institute for Plant & Food Research Ltd, Auckland 1025, New Zealand
3
Plant Health and Environment Laboratory, Investigation and Diagnostic Centre, Ministry for Primary Industries, Biosecurity New Zealand, PO Box 2095, Auckland 1140, New Zealand
4
School of Biological Sciences, The University of Auckland, Private Bag 92019, Auckland 1142, New Zealand
et al. 2016), little has been uncovered on the prevalence of viruses in native plants (Veerakone et al. 2015). Veronica (Syn Hebe; Plantaginaceae) is the largest plant genus in New Zealand, comprising of more than 150 species (136 indigenous (Schönberger et al. 2020)). Exotic species Veronica persica has previously been described as a host of cucumber mosaic virus, watermelon mosaic virus 2, and turnip yellows virus (Fletcher et al. 1999; Fletcher 1989; Kyriakou 1984; Kyriakou et al. 1983). We used a two-step generic approach to optimise the discovery of virus sequences by analysing both double-stranded RNA (dsRNA) and total RNA extracts from four native New Zealand plants. All plants used in this study were cultivated outside as part of the landscape of the Manaaki Whenua Landcare Research campus, Lincoln, New Zealand. Symp
Data Loading...
