First report of tomato leaf curl New Delhi virus infecting Tinda ( Benincasa fistulosa ) in India
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First report of tomato leaf curl New Delhi virus infecting Tinda (Benincasa fistulosa) in India Manmohan Dhkal 1 & Abhishek Sharma 2 & Gurpreet Kaur 2 Received: 20 May 2020 / Accepted: 10 September 2020 # Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2020
Keywords Benincasa fistulosa . Tomato leaf curl New Delhi virus . Yellows and leaf curl . India
In January 2018, severe symptoms of virus(es) and heavy infestation of whitefly (Bemisia tabaci) were observed on Tinda (Benincasa fistulosa) crop grown in vegetable research farm of Punjab Agricultural University. The infected crop symptoms consisted of severe yellowing and curling of leaves. These symptoms resembled to those which were described recently for the infection of tomato leaf curl New Delhi virus (ToLCNDV) on other cucurbit crop in Italy (Trisciuzzi et al. 2018). Eight per cent of 100 plants observed were showing these symptoms. DNA from six symptomatic samples was tested by PCR, with primer pair PALIc and PARIv specific for AV1 region of CP, AC5, AC3, AC2 and some part of AC1 gene of begomovirus (Rojas et al. 1993). This primer pair amplified specific product of 1280 bp in three samples out of six. To further identify the associated begomovirus species, PCR amplicon from one selected sample was cloned in pTZ57R/T vector (Thermo Scientific, USA) and sequenced. The obtained sequences were analyzed by BLASTn tool and compared with existing corresponding sequences of the same viral species available in the GenBank database. Cloned amplicon (MH845616) from infected sample showed 99.5% nucleotide similarity with ToLCNDV (AY939926) infecting Luffa cylindrical in North India and 98.5% sequence similarity with ToLCNDV (HM989845) reported to infect Luffa acutangula at New Delhi. To further confirm this results,
* Manmohan Dhkal [email protected] 1
Department of Plant Pathology, Punjab Agricultural University, 141004 Ludhiana, India
2
Department of Vegetable Science, Punjab Agricultural University, 141004 Ludhiana, India
primer pairs specific for the major tomato leaf curl b eg o m o vi r u s e s viz . t o m a t o l ea f c u r l N e w D e l h i virus (ToLCNDV), tomato leaf curl Gujarat virus (ToLCGV), tomato leaf curl Karnataka virus (ToLCKV) and tomato leaf curl Bangalore virus (ToLCBV) were tested (Reddy et al. 2005) but only primer pair CRNDv30 and CRNDc1181, specific for ToLCNDV, amplified specific product of 1180 bp in all three positive samples thus reconfirming the association of ToLCNDV with samples. To the best knowledge, this is the first report of Benincasa fistulosa plants infected by ToLCNDV. Acknowledgements Authors are thankful to Department of Science and Technology for providing and “Innovation in Science P ursuit for Inspired Research (INSPIRE)” fellowship to Manmohan Dhkal.
References Rojas MR, Gilbertson RL, Russell DR, Maxwell DP (1993) Use of degenerate primers in the polymerase chain reaction to detect whitefly transmitted geminiviruses. Plant Dis 77: 340–347 Trisciuzzi N, Silletti MR, Gallitelli D, Spano R (2018) Fi
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