First report of cucumber mosaic virus infecting Ledebouriella seseloides in Korea
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First report of cucumber mosaic virus infecting Ledebouriella seseloides in Korea Na-Kyeong Kim 1 & Hyo-Jeong Lee 1 & Hwi-Won Jeong 1 & Seon-Min Go 1 & Nam-Yeon Kim 1 & Tae Ho Ryu 1 & Sang-Yun Cho 2 & Mi-Ri Park 3 & Rae-Dong Jeong 1 Received: 3 July 2019 / Accepted: 6 January 2020 # Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2020
Keywords cucumber mosaic virus . Ledebouriella seseloides . Korea
Ledebouriella seseloides (family Apiaceae) is a perennial plant distributed in the coastal areas of Korea, Taiwan, and Northern China. It is commonly used as a folk herbal medicine for antiviral and antibacterial effects, and it is cultivated as a vegetable in kitchen gardens. In April 2019, symptoms caused by a virus, such as obvious mosaic and foliar chlorosis, were observed on plants of L. seseloides at a farm in Samchuck, Korea. To investigate the causative virus, a total of 30 symptomatic leaves were collected from different L. seseloides plants and analyzed using double-antibody sandwich (DAS)-ELISA with polyclonal antibodies against cucumber mosaic virus (CMV), cucumber green mottle mosaic virus, pepper mottle virus, tobacco mosaic virus, tomato mosaic virus, and zucchini yellow mosaic virus (Agdia, Elkhart, IN, USA). The serological analyses showed that none of the samples reacted with any of the antisera, except that 60% of the samples reacted with CMV-specific antibody. To further confirm CMV infection, RT-PCR was conducted with specific primers CPTALL-5′ (5′-YASYTTTDRGGTTCAATTCC-3′) and CPTALL-3′ (5′-GACTGACCATTTTAGCCG-3′) designed to amplify a 945-bp fragment of RNA3, which includes the whole coat protein (CP) gene (Choi et al. 1999). RT-PCR products of the expected size were obtained from 18 symptomatic samples, and these amplicons were cloned and
* Rae-Dong Jeong [email protected] 1
Department of Applied Biology, Institute of Environmentally Friendly Agriculture, Chonnam National University, Gwangju 61185, South Korea
2
Crop Foundation Division, National Institute of Crop Science, Wanju 55365, South Korea
3
Region-Specific Industries Fostering Division, Cheorwon Plasma Research Institute, Gangwon-do 24047, South Korea
subjected to Sanger sequencing. The consensus sequence was deposited in GenBank (accession No. LC476589). Sequence comparison of the complete CP gene (657 nt) revealed that it shared the highest nucleotide identity of 98% with the CP gene of several CMV isolates from Rudbeckia hirta (GU327365), Raphanus sativus (LC066428), Capsicum annuum (KC527751), Cucumis sativus (JN180311), and Musa acuminata (KX216874). The maximum likelihood phylogenetic trees inferred from both the complete CP nucleotide and amino acid sequences generated by MEGA 5.05 revealed that the virus isolate, named CMV-Ls, belongs to the CMV group IA. A host range trial using infected L. seseloides leaf sap obtained from a single lesion of Nicotiana glutinosa infected by CMV-Ls was mechanically inoculated to N. rustica, N. benthamiana, N. glutinosa, and N. tabacum cv. Xanthi nc. Symptoms of infection ap
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