Florfenicol induces renal toxicity in chicks by promoting oxidative stress and apoptosis
- PDF / 2,678,881 Bytes
- 11 Pages / 595.276 x 790.866 pts Page_size
- 80 Downloads / 187 Views
RESEARCH ARTICLE
Florfenicol induces renal toxicity in chicks by promoting oxidative stress and apoptosis Xiao Wang 1 & Chao Han 1 & Yuqing Cui 1 & Yumeng Geng 1 & Yuanyuan Wei 1 & Wanyu Shi 1,2 & Yongzhan Bao 1,2 Received: 27 May 2020 / Accepted: 16 August 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract To explore the mechanism of renal toxicity induced by florfenicol (FFC), 120 chicks were randomly divided into 6 groups, 20 in each group. Except for the control group, different doses of FFC (0.15 g/L, 0.3 g/L, 0.6 g/L, 1.2 g/L, and 1.8 g/L) were added to drinking water in the other 5 groups. Five days later, blood was collected from the vein under the wing, and the complete kidneys were obtained as soon as possible, then tested the experimental indicators. The results showed that compared with control group, all doses of FFC significantly reduced the average weight gain of chicks (P < 0.05 or P < 0.01). Except for the 0.15 g/L FFC group, kidney index of chicks in the other doses of FFC groups were significantly increased (P < 0.05 or P < 0.01). The kidney tissues in all FFC groups showed obvious damage, deformities, cell atrophy, and cell gap enlargement. In addition, all doses of FFC significantly increased the contents of uric acid (UA), blood urea nitrogen (BUN), creatinine (CRE) in serum, and malondialdehyde (MDA) in renal tissue (P < 0.05 or P < 0.01), but significantly reduced the levels of glutathione (GSH), superoxide dismutase (SOD), and catalase (CAT) in renal tissue (P < 0.05 or P < 0.01). FFC significantly inhibited the mRNA and protein expression levels of nuclear factor-erythroid 2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1), and nicotinamide adenine dinucleotide phosphate: quinone oxidoreductase-1 (NQO-1), and increased the mRNA and protein expression levels of p53, Caspase-3, and Caspase-6 (P < 0.05 or P < 0.01). The apoptotic rate of renal cells in all doses of FFC groups increased significantly (P < 0.05 or P < 0.01). It was concluded that FFC had a certain degree of nephrotoxicity, and with the increase of FFC concentration, the kidney injury of chicks became more and more serious. FFC promoted oxidative stress response in kidney of chicks by inhibiting the expression of related factors in Nrf2-ARE pathway. Moreover, the expression of pro-apoptotic factors was upregulated to improve the apoptosis rate of renal cells, which resulted in excessive apoptosis of renal cells and seriously affected the kidney function of chicks. Keywords Florfenicol . Chick . Renal toxicity . Oxidative stress . Apoptosis
Introduction Florfenicol (FFC) is a novel chloramphenicol broadspectrum antibiotic used in veterinary practice to treat Xiao Wang and Chao Han contributed equally to this work. Responsible editor: Mohamed M. Abdel-Daim * Wanyu Shi [email protected] * Yongzhan Bao [email protected] 1
College of Traditional Chinese Veterinary Medicine, Hebei Agricultural University, 2596, Le Kai South Street, Baoding 071001, Hebei, China
2
Hebei Provincial Engineering Techn
Data Loading...
