Mass Cytometry Methods and Protocols
This book details a compilation of up-to-date and cutting-edge protocols in mass cytometry.. Chapters guide readers through setting up a facility, panel design and reagent preparation, sample preparation, specific applications, and data analysis. Written
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Helen M. McGuire · Thomas M. Ashhurst Editors
Mass Cytometry Methods and Protocols
Methods
in
M o l e c u l a r B i o lo g y
Series Editor: John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Mass Cytometry Methods and Protocols
Edited by
Helen M. McGuire Discipline of Pathology, The University of Sydney, Camperdown, NSW, Australia
Thomas M. Ashhurst Sydney Cytometry Facility, The University of Sydney and Centenary Institute, Camperdown, NSW, Australia
Editors Helen M. McGuire Discipline of Pathology The University of Sydney Camperdown, NSW, Australia
Thomas M. Ashhurst Sydney Cytometry Facility The University of Sydney and Centenary Institute Camperdown, NSW, Australia
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-9453-3 ISBN 978-1-4939-9454-0 (eBook) https://doi.org/10.1007/978-1-4939-9454-0 © Springer Science+Business Media, LLC, part of Springer Nature 2019 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors, and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. This Humana Press imprint is published by the registered company Springer Science+Business Media, LLC, part of Springer Nature. The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface Mass cytometry is a state-of-the-art single-cell measurement technology with a multitude of applications. The use of heavy metal-conjugated antibodies allows for the detection of approximately 50 parameters per cell with minimal signal overlap. This has made mass cytometry the mostly highly multi-parametric method for the simultaneous measurement of protein at the single-cell level to date. The strength of this technology lies in its inherent flexibility, from the starting materials that can be examined to the types of markers that can be analyzed and metho
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