Fluorescent and electrochemical dual-mode detection of Chikungunya virus E1 protein using fluorophore-embedded and redox
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ORIGINAL PAPER
Fluorescent and electrochemical dual-mode detection of Chikungunya virus E1 protein using fluorophore-embedded and redox probe-encapsulated liposomes Fahmida Nasrin 1 & Ankan Dutta Chowdhury 2 & Akhilesh Babu Ganganboina 2 & Ojodomo J. Achadu 2 & Farzana Hossain 1 & Masahito Yamazaki 3 & Enoch Y. Park 1,2 Received: 25 August 2020 / Accepted: 18 November 2020 / Published online: 25 November 2020 # Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract The critical goal of sensitive virus detection should apply in the early stage of infection, which may increase the probable survival rate. To achieve the low detection limit for the early stage where a small number of viruses are present in the sample, proper amplified signals from a sensor can make readable and reliable detection. In this work, a new model of fluorescent and electrochemical dual-mode detection system has been developed to detect virus, taking recombinant Chikungunya virus E1 protein (CHIK-VP) as an example. The hydrophobic quantum dots (QDs) embedded in the lipid bilayer of liposome and methylene blue (MB) encapsulated in the inner core of liposomes played a role of dual-signaling modulator. After CHIK-VP addition, the nanocomposites and APTES-coated Fe3O4 nanoparticles (Fe3O4 NPs) were conjugated with antibodies to form a sandwich structure and separated from the medium magnetically. The nanoconjugates have been burst out by chloroform as surfactant, and both the QDs and MB are released from the liposome and were then monitored through changes in the fluorescence and electrochemical signals, respectively. These two fluorometric and electrochemical signals alteration quantified the CHIK-VP in the range of femtogram to nanogram per milliliter level with a LOD of 32 fg mL−1, making this liposomal system a potential matrix in a virus detection platform. Keywords Liposomes . Quantum dots . Biosensor . Chikungunya virus protein . Fluorescence detection . Electrochemical detection
Introduction Virus detection with higher sensitivity and selectivity, along with the presence of other interferences, is of great importance
to control the annual epidemic [1–5]. In most viral diseases, the progress of vaccination is a time-consuming process that encourages the necessity for the development of rapid sensing technologies to prevent the viral outbreak. The rapid
Fahmida Nasrin and Ankan Dutta Chowdhury contributed equally to this work. * Enoch Y. Park [email protected] Fahmida Nasrin [email protected]
Masahito Yamazaki [email protected]
1
Laboratory of Biotechnology, Graduate School of Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan
2
Laboratory of Biotechnology, Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan
3
Research Institute of Electronics, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan
Ankan Dutta Chowdhury [email protected] Akhil
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